Process for the production of human urokinase
AssigneeKabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
InventorKaname Sugimoto
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Abstract
The present invention relates to a process for the production of human urokinase. More precisely, the present invention relates to a process for the mass production of human urokinase, comprising in vivo multiplication of human cells capable of producing human urokinase, using the nutrient body fluid of a non-human warm-blooded animal, and exposure of the multiplied human cells to an urokinase inducer. The human urokinase present production according to the invention is much higher than that attained by conventional methods; thus, human urokinase can be used in sufficient amount in the prevention and treatment of human diseases.
Claims22
A process for producing human urokinase, comprising:
- fusing parent human cells inherently capable of producing human urokinase with a human lymphoblastoid line to obtain hybridoma cells capable of producing human urokinase;
- implanting said hybridoma cells in an immunodeficient or immunosuppressed non-human warm-blooded animal;
- feeding the animal to allow said hybridoma cells to utilize the nutrient body fluid of the animal for their multiplication;
- extracting and disaggregating the resultant tumor, formed in the animal, to obtain the multiplied hybridoma cells;
- culturing the multiplied hybridoma cells on an in vitro nutrient medium in the presence of an effective amount of a urokinase inducer under conditions appropriate to accumulate a substantial amount of human urokinase;
- and
- recovering the accumulated human urokinase from the culture.
A process for producing human urokinase, comprising:
- fusing parent human cells inherently capable of producing human urokinase with a human lymphoblastoid line to obtain hybridoma cells capable of producing human urokinase;
- suspending said hybridoma cells in a diffusion chamber capable of permitting the nutrient body fluid of a non-human warm-blooded animal to to supplied to said hybridoma cells;
- embedding or placing said chamber in or on a non-human warm-blooded animal in a manner such that the nutrient body fluid of said animal is supplied to the cells within said chamber;
- feeding said animal to allow said hybridoma cells to utilize the nutrient body fluid for their multiplication;
- collecting the multiplied hybridoma cells from said chamber;
- culturing the multiplied hybridoma cells on an in vitro nutrient medium in the presence of an effective amount of a urokinase inducer under conditions appropriate to accumulate a substantial amount of human urokinase;
- and
- recovering the accumulated human urokinase from the culture.
Dependencies are inferred from the claim text.