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US 4,537,774 AGranted
Hot-water extracts of neem bark
AssigneeTerumo Kabushiki Kaisha
InventorsMasaki Shimizu, Tadashi Sudo, Takeo Nomura
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AssigneeTerumo Kabushiki Kaisha
InventorsMasaki Shimizu, Tadashi Sudo, Takeo Nomura
Estimating the term…
Hot-water extracts of the neem bark produced by treating the bark of neem with water at a temperature from 0.degree. to 40.degree. C. and subjecting the residue from said pre-extraction treatment to extraction treatment with hot water. Purification of the extract thus produced by means of alcohol precipitation or dialysis gives products of a higher purity. Moreover, treatments prior to the pre-extraction treatment with water at 0.degree.-40.degree. C. with a non-polar organic solvent having a dielectric constant of 10 or lower and a polar organic solvent having a dielectric constant from 15 to 35 yields neem extracts of a further higher purity. The hot-water extracts of the neem bark according to the present invention possess activity against mouse L-5178Y cells and transplanted sarcoma 180 tumors.
Hot-water extract of neem bark which is characterized by being active against mouse L-5178Y cells and against transplanted sarcoma 180 tumors produced by successively contacting the bark of neem which is Melia azadirachta with a polar organic solvent selected from the group consisting of an alcohol, pyridine and acetone, and water at a temperature from 0.degree. C. to 40.degree. C. to form liquid extracts and a residue, subjecting said residue from said treatments to extraction with hot water at the boiling temperature to obtain hot-water liquid extract and purifying said hot water liquid extract by alcohol precipitation or dialysis using a diaphragm which fractionates molecules having a molecular weight of 50,000 or lower.
Hot-water extract of neem bark which is characterized by being active against mouse L-5178Y cells and against transplanted sarcoma 180 tumors produced by successively contacting the bark of neem which is Melia azadirachta with a non-polar organic solvent selected from the group consisting of benzene, toluene, xylene, n-hexane, chloroform, carbon tetrachloride and ethyl acetate, with a polar organic solvent selected from the group consisting of an alcohol, pyridine and acetone, and with water at a temperature from 0.degree. C. to 40.degree. C. to form liquid extracts and a residue, subjecting said residue from said treatments to extraction with hot water at the boiling temperature to obtain a hot-water liquid extract and purifying said hot-water liquid extract by alcohol precipitation or dialysis using a diaphragm which fractionates molecules having a molecular weight of 50,000 or lower.
Dependencies are inferred from the claim text.