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US 4,536,479 AGranted
Use of anti-idiotype antibodies in immunoassays
AssigneeE. I. Du Pont de Nemours and Company
InventorRonald Vander-Mallie
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Abstract
The use of anti-idiotype antibodies as functional substitutes for antigens or haptens in immunoassays is disclosed.
Claims4
A homogeneous immunoassay for the detection of a nonimmunoglobulin analyte in a test sample, comprising:
- (1) contacting the test sample with reagents comprising:
- (a) a first covalent conjugate comprising a first enzyme and an idiotypic anti-analyte antibody;
- (b) a second covalent conjugate comprising a second enzyme and an anti-idiotypic antibody capable of competing with the analyte for the idiotypic anti-analyte antibody;
- (c) a substrate for the first and or second enzyme wherein one of the enzymes and the substrate are capable of reacting to produce a first product, which product is a substrate for the other enzyme, which is capable of reacting with the first product to produce a second, detectable product;
- and
- (2) measuring the amount of second product which is inversely related to the amount of analyte initially present in the test sample.
3 more elements
A homogeneous immunoassay for the detection of a nonimmunoglobulin analyte in a test sample comprising:
- (1) forming a reaction mixture by contacting the test sample with reagents, comprising:
- (a) a first covalent conjugate comprising a
- first fluorophore and an idiotypic anti-analyte antibody;
- and
- (b) a second covalent conjugate comprising a second fluorophore and an anti-idiotypic antibody capable of competing with the analyte for the idiotypic anti-analyte antibody;
- wherein one of the fluorophores is capable of absorbing incident light at a wavelength .lambda..sub.inc to produce light emission at wavelength .lambda..sub.em.sbsb.1 which can be absorbed by the other fluorophore to produce emission at wavelength .lambda..sub.em.sbsb.2 ;
- (2) irradiating the reaction mixture with light of wavelength .lambda..sub.inc ;
- and
- (3) measuring the intensity of light of wavelength .lambda..sub.em.sbsb.1 or .lambda..sub.em.sbsb.2, which intensity is related to the amount of analyte initially present in the test sample.
6 more elements
Dependencies are inferred from the claim text.