Method for reducing non-specific interferences in agglutination immunoassays
AssigneeSeragan Diagnostics, Inc.
InventorsRoger L. Sokoloff, John M. Reno
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Abstract
An agglutination immunoassay in which non-specific interferences are reduced by adding to the reaction mixture a halogen substituted carboxylic acid such as chloroacetic acid, dichloroacetic acid, trichloroacetic acid, bromoacetic acid, dibromoacetic acid, tribromoacetic acid, iodoacetic acid and mixtures thereof.
Claims5
In an agglutination immunoassay for an analyte in a fluid sample which is combined with a reagent which includes sensitized latex particles and binding partner as required, whereby the degree of agglutination of the particles is a measure of the analyte in the sample, the improvement comprising the step of adding to the reaction mixture an additive to decrease non-specific interferences of the agglutination of the particles, where such additives comprise at least one halogen substituted carboxylic acid or water soluble salt thereof wherein the acid has the formula:
- ##STR2## and wherein R.sub.1 is Cl, Br or I and R.sub.2 is H, Cl, Br or I and R.sub.3 is H, CH--.sub.3 --, CH.sub.3 CH.sub.2 --, Cl, Br or I.
Dependencies are inferred from the claim text.