Thermostable glucoamylase and method for its production
AssigneeCPC International Inc.
InventorsDennis M. Katkocin, Nancy S. Word, Shiow-Shong Yang
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Abstract
This invention relates to a glucoamylase enzyme exhibiting thermostability at pH values between 6 and 7 which is derived from a spore-forming, thermophilic, anaerobic bacterium and to a process for its production. The glucoamylase is especially useful for the preparation of glucose-containing syrups from starch.
Claims9
A glucoamylase enzyme derived from a Clostridium thermoamylolyticum microorganism, said enzyme having a molecular weight of about 75,000.+-.3,000 as determined by SDS-polyacrylamide gel electrophoresis, having a half-life of greater than 3 hours at pH 6 and 70.degree. C., having a maximum glucoamylase activity at a pH of about 5.0, and having a maximum glucoamylase activity at pH 5 at a temperature of about 70.degree.-75.degree. C.
A glucoamylase enzyme derived from a microorganism selected from the group consisting of Clostridium sp. ATCC No. 39,251, ATCC No. 39,252, and mutant strains thereof, said glucoamylase having a half-life greater than 3 hours at pH 6 and 70.degree. C.
A process for producing a glucoamylase enzyme having a molecular weight of about 75,000.+-.3,000 as determined by SDS-polyacrylamide gel electrophoresis, having a half-life of greater than 3 hours at pH 6 and 70.degree. C., having a maximum glucoamylase activity at a pH of about 5.0, and having a maximum glucoamylase activity at pH 5 at a temperature of about 70.degree.-75.degree. C., which comprises culturing cells of a strain of Clostridium thermoamylolyticum in a nutrient medium and then isolating the glucoamylase enzyme from the medium.
A process for producing a glucoamylase enzyme which comprises selecting a microorganism from the group consisting of Clostridium sp. ATCC No. 39,251, ATCC No. 39,252, and mutant strains thereof, culturing cells of the selected microorganism in a nutrient medium and then isolating the glucoamylase enzyme from the medium.
Dependencies are inferred from the claim text.