Absorbance monitor

A method comprising the steps of:

• focusing light with a mirror from a small spot on the light emitting portion of a light source through an aperture in an aperture plate;
• splitting the light passing through the aperture plate into two beams of light without creating a polarization difference between the two beams of light;
• focusing one of said beams of light within a small aperture and using it to sense the characteristic of a sample and focusing the said other beam of light through an aperture of the same size with both apertures being of such a size as to contain within each of them the image of the aperture in the aperture plate, wherein said other beam of light serves as a reference;
• converting the beams of light into electrical signals after said one beam of light has been brought into intimate contact with the sample and comparing the signals to cancel common-mode noise;
• and
• converting said signals to the log form of the amplitude of the signals and feeding back at least the log of one of the signals to the light source to stabilize the light source.

A method comprising the steps of:

• focusing light from a small spot on the light emitting portion of a light source through an aperture in an aperture plate;
• splitting the light passing through the aperture plate into two beams of light without creating a polarization difference in the two beams of light;
• focusing one of said beams of light within a small aperture and using it to sense the characteristic of a sample and focusing the said other beam of light through an aperture of the same size with both apertures being of such a size as to contain within each of them the image of the aperture in the aperture plate, wherein said other beam of light serves as a reference;
• converting said sensing signal and said reference signal into electrical signals after said one beam of light has been brought into intimate contact with the sample and comparing said electrical signals to cancel said common-mode noise;
• and
• converting said signals to the log form of the amplitude of the signals and feeding back at least the log of one of the signals to the light source to stabilize the light source.

A method comprising the steps of:

• focusing light from a small spot on the light emitting portion of a light source through an aperture in an aperture plate;
• splitting the light passing through the aperture plate into two beams of light without creating a polarization difference between the two beams of light;
• focusing one of said beams of light within a small aperture and using it to sense the characteristic of a sample and focusing the said other beam of light through an aperture of the same size with both apertures being of such a size as to contain within each of them the image of the aperture in the aperture plate, wherein said other beam of light serves as a reference;
• comparing the signals resulting from said beams of light after said one beam of light has been brought into intimate contact with the sample to cancel common-mode noise;
• and
• converting said signals to the log form of the amplitude of the signals and feeding back at least the log of one of the signals to the light source to stabilize the light source.

A method comprising the steps of:

• focusing light from a small spot on the light emitting portion of a light source through an aperture in an aperture plate;
• splitting the light passing through the aperture plate into two beams of light;
• focusing one of said beams of light within a small aperture and using it to sense the characteristic of a sample and focusing the said other beam of light through an aperture of the same size with both apertures being of such a size as to contain within each of them the image of the aperture in the aperture plate, wherein said other beam of light serves as a reference;
• comparing the signals resulting from said beams of light after said one beam of light has been brought into intimate contact with the sample to cancel common-mode noise;
• and
• converting said signals to the log form of the amplitude of the signals and feeding back at least the log of one of the signals to the light source to stabilize the light source.

A method comprising the steps of:

• focusing light from a small spot on the light emitting portion of a light source through an aperture in an aperture plate;
• splitting the light passing through the aperture plate into two beams of light;
• using one of said beams of light to sense a characteristic of a sample and using the other beam of light as a reference;
• comparing the signals resulting from said beams of light after said one beam of light has been brought into intimate contact with the sample to cancel common-mode noise;
• and
• converting said signals to the log form of the amplitude of the signals and feeding back at least the log of one of the signals to the light source to stabilize the light source.

A method comprising the steps of:

• focusing light from a small spot on a light source into a beam of light;
• splitting the beam of light into two beams of light;
• bringing one of said two beams of light into intimate contact with a sample;
• converting each of the two beams of light into electrical signals having amplitudes which are the log of the intensity of the beams of light;
• feeding at least one of said signals back to the light source to control fluctuations in the light source;
• and
• comparing the two signals resulting from said two beams of light after said one beam of light has been brought into intimate contact with the sample to cancel common-mode noise.

A method comprising the steps of:

• focusing light from a small spot on the light emitting portion of a light source into a beam;
• splitting the light in said beam into two beams of light without creating substantial polarization of the light in either beam;
• using one of said beams of light to sense a characteristic of a sample and using the other beam of light as a reference;
• comparing the signals resulting from said two beams of light after said one beam of light has been brought into intimate contact with the sample to cancel common-mode noise;
• and
• converting said signals to the log form of the amplitude of the signal and feeding back at least the log of one of the signals to the light source to stabilize the light source.

Apparatus comprising:

• a light source;
• focusing means for focusing light from a first small spot on the light emitting portion of said light source onto a second small spot;
• an aperture plate having a relatively small aperture in it;
• said aperture plate being placed at said location where said aperture in it coincides with said second small spot;
• said light source comprising a gas discharge lamp;
• said gas discharge lamp having a central bright spot;
• said focusing means including means for focusing light from at least a portion of the central bright spot of said gas discharge lamp onto said second small spot;
• said focusing means including an aspheric concave mirror;
• means for forming a beam of light from the light passing through said aperture and splitting said beam of light into first and second beams of light without substantial polarization of the light;
• means for bringing said first of said beams of light into intimate contact with a sample;
• means for converting said first of said beams of light into a first electrical signal after it has been brought into intimate contact with a sample;
• means for converting the second of said beams of light into a second electrical signal;
• means for comparing said first and second electrical signals to remove common-mode noise;
• said means for splitting said beam of light into two beams of light including a quartz beam splitter positioned at an angle of approximately ten degrees from the incident light;
• means for converting said first and second electrical signals into signals having an amplitude which is the log of the amplitude of said first and second signals;
• and
• feedback means for feeding the log of one of said first and second signals back to said light source for regulating the intensity thereof.

Apparatus comprising:

• a light source;
• focusing means for focusing light from a first small spot on the light emitting portion of said light source onto a second small spot;
• an aperture plate having a relatively small aperture in it;
• said aperture plate being placed at said location where said aperture in it coincides with said second small spot;
• means for forming a beam of light from the light passing through said aperture and splitting said beam of light into first and second beam of light without substantial influence upon the polarization of the light;
• means for bringing said first of said beams of light into intimate contact with a sample;
• means for converting said first of said beams of light into a first electrical signal after it has been brought into intimate contact with a sample;
• means for converting the second of said beams of light into a second electrical signal;
• comparing said first and second electrical signals to remove common-mode noise;
• said means for splitting said beam of light into two beams of light including a quartz beam splitter positioned at an angle of approximately ten degrees from the incident light;
• said means for splitting a beam of light including aperture stops on a flow cell and an aperture plate;
• said aperture stops and aperture plate having apertures in them for receiving respectively said first beam of light and said second beam of light;
• said aperture stops and apertures being of such a size as to have the image of said aperture in said aperture plate fall within each of them;
• means for converting said first and second electrical signals into signals having an amplitude which is the log of the amplitude of said first and second signals;
• and
• feedback means for feeding the log of one of said first and second signals back to said light source for regulating the intensity thereof.

Apparatus comprising:

• a light source;
• focusing means for focusing light from a first small spot on the light emitting portion of said light source onto a second small spot;
• an aperture plate having a relatively small aperture in it;
• said aperture plate being placed at said location where said aperture in it coincides with said second small spot;
• means for forming first and second beams of light from the light passing through said aperture;
• means for bringing said first of said beams of light into intimate contact with a sample;
• means for converting said first of said beams of light into a first electrical signal after it has been brought into intimate contact with a sample;
• means for converting the second of said beams of light into a second electrical signal;
• means for comparing said first and second electrical signals to remove common-mode noise;
• said means for splitting said beam of light into two beams of light including a quartz beam splitter positioned at an angle of approximately ten degrees from the incident light;
• said means for forming including aperture stops on a flow cell and an aperture plate;
• said aperture stops and aperture plate have apertures in them for receiving respectively said first beam of light and said second beam of light;
• said aperture stops and apertures being of such a size as to have the image of said aperture and said aperture plate fall within each of them;
• means for converting said first and second electrical signals into signals having an amplitude which is the log of the amplitude of said first and second signals;
• and
• feedback means for feeding the log of one of said first and second signals back to said light source for regulating the intensity thereof.

Apparatus comprising:

• a light source;
• focusing means for focusing light from a first small spot on said light source onto a second small spot;
• an aperture plate having a relatively small aperture in it;
• said aperture plate being placed at a location where said aperture in it coincides with said second small spot;
• means for splitting the light passing through said aperture into two beams of light;
• said means for splitting the light passing through said aperture including a quartz beam splitter positioned with its surface at an angle of approximately 10 degrees to the normal to the direction of the light passing through the aperture;
• means for converting said two beams of light into first an second electrical signals;
• means for converting one of said first and second electrical signals into a third electrical signal having an amplitude which is the log of the amplitude of said one of said first and second signals;
• and
• feedback means for feeding said third signal back to said light source for regulating the intensity thereof.

Apparatus comprising a light source;

• focusing means for focusing light from a first small spot on said light source onto a second small spot;
• an aperture plate having a relatively small aperture in it;
• said aperture plate being placed at a location where said aperture in it coincides with said second small spot;
• means for splitting the light passing through said aperture into first and second beams of light;
• means for converting said first and second beams of light into first and second electrical signals;
• means for converting one of said first and second electrical signals into a third electrical signal having an amplitude which is the log of the amplitude of said one of said first and second electrical signals;
• and
• feedback means for feeding the log of said third electrical signal back to said light source for regulating the intensity thereof.

Apparatus comprising:

• a light source;
• means for forming a beam of light from the light in said light source;
• means for splitting said beam of light into first and second beams of light without substantial alteration of the polarization of the light;
• means for using said first and second beams of light to measure a characteristic of a substance;
• means for bringing said first of said beams of light into intimate contact with a sample;
• means for converting said first of said beams of light into a first electrical signal after it has been brought into intimate contact with a sample;
• means for converting the second of said beams of light into a second electrical signal;
• means for comparing said first and second electrical signals to remove common-mode noise;
• said means for splitting said beam of light into two beams of light including a quartz beam splitter positioned at an angle of approximately ten degrees from the incident light;
• said means for splitting a beam of light including aperture stops on a flow cell and an aperture plate;
• said aperture stops and aperture plate having apertures in them for receiving respectively said first beam of light and said second beam of light;
• said aperture stops and apertures being of such a size as to have the image of said aperture and said aperture plate fall within each of them;
• means for converting said first and second electrical signals into signals having an amplitude which is the log of the amplitude of said first and second signals;
• and
• feedback means for feeding the log of one of said first and second signals back to said light source for regulating the intensity thereof.

Apparatus comprising:

• a light source;
• means for forming a beam of light from the light in said light source;
• means for splitting said beam of light into first and second beams of light without substantial alteration of the polarization of the light;
• means for using said first and second beams of light to measure a characteristic of a substance;
• said means for splitting said beam of light into two beams of light including a quartz beam splitter positioned at an angle of approximately ten degrees from the incident light and aperture stops on a flow cell and an aperture plate;
• said aperture stops and aperture plate having apertures in them for receiving respectively said first beam of light and said second beam of light;
• said aperture stops and apertures being of such a size as to have the image of said aperture and said aperture plate fall within each of them;
• means for converting said first and second electrical signals into signals having an amplitude which is the log of the amplitude of said first and second signals;
• and
• feedback means for feeding the log of one of said first and second signals back to said light source for regulating the intensity thereof.

Apparatus comprising:

• a light source;
• means for forming a beam of light from the light in said light source;
• means for splitting said beam of light into first and second beams of light without substantial alteration of the polarization of the light;
• means for using said first and second beams of light to measure a characteristic of a substance;
• said means for splitting a beam of light including aperture stops on a flow cell and an aperture plate;
• said aperture stops and aperture plate having apertures in them for receiving respectively said first beam of light and said second beam of light;
• said aperture stops and apertures being of such a size as to have the image of said aperture of said aperture plate fall within each of them;
• means for converting said first and second electrical signals into signals having an amplitude which is the log of the amplitude of said first and second signals;
• and
• feedback means for feeding the log of one of said first and second signals back to said light source for regulating the intensity thereof.

Apparatus comprising:

• a light source;
• means for forming a beam of light from the light emitted by said light source;
• means for splitting said beam of light into first and second beams of light;
• means for bringing said first beam of light into intimate contact with a sample;
• means for converting said first beam of light into a first electrical signal after it has been brought into intimate contact with a sample;
• means for converting said second beam of light into a second electrical signal;
• means for converting said first and second electrical signals into signals having an amplitude which is the log of the amplitude of said first and second signals;
• means for feeding back the log signal of one of said first and second signals to said source of light to control the intensity of said source of light;
• and
• means for comparing the logs of said first and second electrical signals to remove common-mode noise.