Tissue culture medium
AssigneeAMF Incorporated
InventorsReynaldo L. Maldonado, Kenneth A. Rosanoff
Life of this patent
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Abstract
A growth factor containing serum is disclosed which is derived from natural bovine serum is disclosed which is by precipitating a first solids fraction from natural bovine serum by adding ammonium sulfate to about 25% saturation to give a first solids fraction and a first supernatant; separating the first solids fraction from the first supernatant; precipitating a second solids fractions from the first supernatant by adding ammonium sulfate to about 40% saturation to give a second solids fraction and a second supernatant; separating the second solids fraction from the second supernatant; combining the first solids fraction and the second supernatant; and desalinating the first solids fraction and the second supernatant, either before or after combination thereof.
Claims9
A process for producing a growth factor containing serum having a .gamma.-globulin content of between about 0.1-1.0 g/dl, and of 50-100 mg/dl of cholesterol comprising the steps of:
- (a) adding ammonium sulfate to natural bovine serum to 15-30% saturation to give a first solids fraction and a first supernatant;
- (b) separating said first solids fraction from said first supernatant;
- (c) adding ammonium sulfate to said first supernatant to 35-45% saturation to give a second solids fraction and a second supernatant;
- (d) separating said second solids fraction from said second supernatant;
- (e) combining said first solids fraction and said second supernatant;
- and
- (f) desalinating said first solids fraction and said second supernatant, either before or after said combining.
Dependencies are inferred from the claim text.