Novel proteinous substance KUD-PC and its production

A novel protein KUD-PC having the following physicochemical properties:

• (A) Elementary analysis:
• comprising carbon, hydrogen, nitrogen, oxygen and sulfur;
• C 49.98%, H 7.27%, N 15.23%, S 1.08%,
• (B) Molecular weight:
• 11,500 (SDS-polyacrylamide gel electrophoresis),
• (C) Melting point:
• 258.degree.-260.degree. C. (decomp.),
• (D) Specific rotation:
• [.alpha]sub.D.sup.20 =-55.8.degree. (c=1.0, H.sub.2 O),
• (E) Ultraviolet absorption spectrum:
• as shown in FIG. 1,
• (F) Infrared absorption spectrum:
• as shown in FIG. 2,
• (G) Solubility:
• soluble in water, insoluble in organic solvent (alcohol, acetone and benzene),
• (H) Color reaction:
• positive:
• Folin-Lowry, biuret and Rydon-Smith reactions, negative:
• phenol-H.sub.2 SO.sub.4 and anthrone-H.sub.2 SO.sub.4 reactions, HCl hydrolysate:
• positive for ninhydrin reaction,
• (I) Nature:
• pH 4-8 in 0.1% aqueous solution,
• (J) Color and crystalline form:
• colorless crystals,
• (K) Amino acid composition:
• Molar ratio of amino acid analysis of HCl hydrolysate is as follows;
• ______________________________________ aspartic acid 0.144 threonine 0.258 serine 0.226 glutamic acid 0.141 proline 0.111 glycine 0.250 alanine 0.363 valine 0.265 isoleucine 0.016 leucine 0.117 methinine -- tyrosine 0.020 phenylalanine 0.079 lysine 0.089 arginine 0.040 histidine (trace) ______________________________________
• (L) Electrophoresis:
• Electrophoretic profile of SDS-polyacrylamide gel electrophoresis is shown in FIG. 3 and the substance shows single peak.