Back to results
US 4,528,265 AGranted
Processes and products involving cell modification
OwnerRobert O. Beckerinventor
Life of this patent
Estimating the term…
Abstract
Living mammalian cells are modified by subjecting them to the influence of the ions from a metal electrode, for example of silver, which is placed in contact with them and which is made electrically positive, and causing a low intensity direct current to flow through them. The cells, which are relatively specialized, such as normal mammalian fibroblasts, assume a simpler, relatively unspecialized form and come to resemble hematopoetic or marrow-like cells.
Claims24
A cell modification process which comprises the steps of:
- (a) providing an element of metal at a culture medium having cells of a mammal therein including transformable cells chosen from the group consisting of fibroblast cells and malignant cells;
- (b) causing the element to introduce ions of the metal to the medium, the ions being substantially free of anions;
- (c) contacting the cells with the ions;
- (d) maintaining said contact for a sufficient period of time and with a sufficient amount of the ions to change the transformable cells to unspecialized cells characterized by their relatively multi-potent state;
- said period of time being greater than the time normally necessary to inhibit or render bacteriostatic bacteria in a wound with respect to fibroblast cells, and being less than the time normally necessary to kill Erlich's ascites fluid tumor cells with respect to free-floating malignant cells;
- (e) permitting or inducing a desired number of unspecialized cells to redifferentiate into cells of the culture medium other than fibroblast cells or malignant cells.
3 more elements
A cell modification process which comprises the steps of:
- (a) providing an element of metal consisting essentially of silver which is connected to an anode of a source of direct current and a cathode thereof is connected at a culture medium having cells of a mammal therein including transformable cells chosen from the group consisting of fibroblast cells and malignant cells;
- (b) causing the element of metal to introduce ions of the metal to the medium, the ions being substantially free of anions;
- (c) contacting the cells with the ions;
- and
- (d) maintaining said contact for a sufficient period of time and with a sufficient amount of the ions to change the cells to unspecialized cells characterized in having a relatively multipotent state;
- said period of time being greater than the time normally necessary to inhibit or render bacteriostatic bacteria in a wound with respect to fibroblast cells, and being less than the period of time normally necessary to kill Erlich's ascites fluid tumor cells with respect to free-floating malignant cells;
- (e) permitting or inducing a desired number of unspecialized cells to redifferentiate into cells of the culture medium other than fibroblast cells or malignant cells.
4 more elements
A cell production process which comprises the steps of:
- (a) providing an element of metal at a lesion of a mammal, the lesion providing a first culture medium having fibroblast cells of the mammal therein;
- (b) causing the element of metal to introduce ions of the metal to the medium, the ions being substantially free of anions;
- (c) contacting the cells with the ions;
- (d) maintaining said contact for a sufficient period of time and with a sufficient amount of the ions as to cause production, in the lesion, of granulation tissue having fibroblast cells and unspecialized cells characterized by their multipotent state therein;
- and
- (e) introducing an explant of the granulation tissue from the lesion containing fibroblast cells to a second culture medium in the presence of silver ions such that the fibroblast cells produce unspecialized cells at an accelerated rate.
3 more elements
A cell modification control process which comprises the steps of:
- (a) providing an element of metal at a culture medium having cells of a mammal therein chosen from the group consisting of fibroblast cells and malignant cells, the element being connected to an anode of a source of direct current and the medium being connected to a cathode thereof, thereby causing the element to introduce ions of the metal to the medium and into contact with the cells for a sufficient period of time to modify the cells into unspecialized cells characterized by their relatively multipotent state and free-floating nature, the ions being substantially free of anions;
- said sufficient period of time being greater than the time normally necessary to inhibit or render bacteriostatic bacteria in a wound with respect to fibroblast cells, and being less than the time normally necessary to kill Erlich's ascites fluid tumor cells with respect to free-floating malignant cells;
- and
- (b) thereafter maintaining such modified, unspecialized cells in the medium in the presence of the ions to preserve the modified, unspecialized cells in their modified state.
1 more element
A tissue regeneration process which comprises the steps of:
- (a) providing an element of metal at a lesion of a mammal, the lesion providing a first culture medium having fibroblast cells of a mammal therein;
- (b) causing the element of metal to introduce ions of the metal to the medium, the ions being substantially free of anions;
- (c) contacting the cells with the ions;
- (d) maintaining said contact for a sufficient period of time and with a sufficient amount of the ions as to cause production, in the lesion, of granulation tissue having fibroblast cells and unspecialized cells characterized by their multipotent state therein;
- (e) introducing an explant of the granulation tissue from the lesion containing fibroblast cells to a second culture medium in the presence of silver ions such that the fibroblast cells produce unspecialized cells at an accelerated rate;
- and
- (f) introducing the produced unspecialized cells into a third culture medium containing a specific mammalian tissue composed of a specific kind of cell and permitting or inducing the unspecialized cell to become differentiated into the type of cell that composes the tissue.
4 more elements
Dependencies are inferred from the claim text.