Purification and activity assurance of precipitated heterologous proteins
AssigneeGenentech, Inc.
InventorKenneth C. Olson
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Abstract
A process for isolating and purifying a precipitated heterologous protein from a host cell culture, including treating the host cell culture with a buffered solution of ionic strength suitable to solubilize most of the host protein but in which refractile heterologous protein is substantially insoluble, and disrupting the cells to form a supernatant and an insoluble fraction; treating the insoluble fraction with a strongly denaturing solution to solubilize the refractile heterologous protein; and recovering renatured heterologous protein.
Claims11
A process for isolating and purifying a precipitated heterologous protein from host cell culture, which process comprises:
- (a) treating the host cell culture with a buffered solution of ionic strength suitable to solubilize most of the host protein, but in which refractile heterologous protein is substantially insoluble and disrupting the cells, to form a supernatant and an insoluble fraction;
- (b) treating the insoluble fraction from step (a) with a strongly denaturing solution to solubilize the refractile heterologous protein, and
- (c) recovering renatured heterologous protein from the solution of Step (b).
Dependencies are inferred from the claim text.