Molecular separation column and use thereof
AssigneeAMF Incorporated
InventorsAlvin L. Crowder, III, Nils L. Dailey, Joseph V. Fiore, Kenneth C. Hou
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Abstract
A molecular separation column for effecting the differential distribution, between two phases, of the components of a sample flowing therethrough. The column contains a substantially homogenous solid stationary phase which comprises a porous matrix of fiber having particulate immobilized therein, wherein at least one of said fiber or particulate is effective for molecular separation. The column is characterized by a reduced pressure drop, increased axial dispersion, more uniform peak shapes and better separations at high sample loading.
Claims30
A chromatography column for effecting differential distribution between two phase, of the components of a sample flowing therethrough, said column containing a solid stationary phase substantially homogeneous with respect to each component thereof, which comprise a porous matrix of fiber having particulate immobilized therein, at least one of said fiber or particulate therein having chromatographic functionality and being effective for chromatographic separation, wherein said solid stationary phase cooperates with the interior wall of the column to form a substantially fluid tight seal therewith, preventing any appreciable skewing or by-pass of fluid around the solid stationary phase.
In a method of effecting a chromatographic separation by effecting a differential distribution of a sample's components between two phases by passing a mobile phase through a chromatography column containing a solid stationary phase, the improvement which comprises employing as said stationary phase a porous matrix of fiber having particulate immobilized therein, at least one of said fiber or particulate having chromatographic functionality and being effective for chromatographic separation, said solid stationary phase being substantially homogeneous with respect to each component thereof, wherein said solid stationary phase cooperates with the interior wall of the column to form a substantially fluid tight seal therewith, preventing any appreciable skewing or bypass of fluid around the solid stationary phase.
Dependencies are inferred from the claim text.