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US 4,511,503 AGranted
Purification and activity assurance of precipitated heterologous proteins
AssigneeGenentech, Inc.
InventorsKenneth C. Olson, Rong-Chang Pai
Life of this patent
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Abstract
A process for dissolving refractile proteins from their insoluble form by using a strongly denaturing solution.
Claims2
A method of solubilizing heterologous protein, produced in an insoluble "refractile form in a recombinant host cell culture, which method comprises:
- isolating and insoluble "refractile" heterologous protein from said host cell culture, and
- treating said protein with a strongly denaturing solution selected from the group consisting of a guanidine salt or a salt of thiocyanate having a concentration between about 4M and 9M to solubilize said refractile protein.
Dependencies are inferred from the claim text.