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US 4,511,502 AGranted
Purification and activity assurance of precipitated heterologous proteins
AssigneeGenentech, Inc.
InventorsStuart E. Builder, John R. Ogez
Life of this patent
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Abstract
A process for purifying heterologous proteins from higher molecular weight components, including dissolving the heterologous protein in a strong denaturing solution and removing the higher molecular weight components using a molecular sieve or high speed centrifugation.
Claims12
A process for purifying heterologous proteins which are expressed and deposited in insoluble, refractile form in a host cell culture, which process includes the steps of:
- (a) isolating said insoluble refractile heterologous protein from said host cell culture;
- (b) dissolving the isolated refractile heterologous protein in a strong denaturing solution;
- followed by
- (c) removing high molecular weight impurities using a molecular sieve or high speed centrifugation techniques.
1 more element
Dependencies are inferred from the claim text.