Apparatus and assay method for the quantitative determination of mineral content in bone
AssigneeThe Medical College of Wisconsin, Inc.
InventorCharles E. Brown
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Abstract
An assay method for the quantitative determination of the mineral content of bone comprises using proton decoupling and magic angle sample spinning techniques to record well resolved .sup.31 P nuclear magnetic resonance spectra of unfractionated bone biopsy samples and characterizing the differences in mineral identity and content from those of normal bone. A rotor for performing the assay includes a compartment for the sample to be assayed, and a separate compartment for a reference material.
Claims4
A rotor for an NMR spectrometer, said rotor having a cone shaped nose portion in a main body portion, said main body portion having a sample compartment for receiving a sample to be analyzed, such as unfractioned bone, a removable cover for the sample container and a reference material compartment, said sample and reference material compartment being positioned end to end along a longitudinal axis and being separated by a wall.
An assay for the mineral content in bone which comprises identifying and quantifying mineral forms in the bone by placing a sample of the bone in a rotor for an NMR spectrometer, which rotor contains a reference material, and then using magic angle sample spinning and dipolar decoupling techniques to record well resolved .sup.31 P NMR spectra of unfractionated bone biopsy samples and comparing the spectral results for the bone with those of the reference material.
Dependencies are inferred from the claim text.