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US 4,510,251 AGranted
Fluorescent polarization assay for ligands using aminomethylfluorescein derivatives as tracers
AssigneeAbbott Laboratories
InventorsCurtis L. Kirkemo, Mohammed T. Shipchandler
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Abstract
This disclosure relates to a method for determining ligands in biological fluids such as serum, plasma, spinal fluid, amnionic fluid and urine, wherein a novel class of aminomethylfluorescein derivative tracer compounds are employed as reagents in fluorescence polarization immunoassays.
Claims5
A method for determining ligands in a sample comprising intermixing with said sample a tracer of the formula:
- ##STR14## wherein R.sub.1 is a ##STR15## group wherein R" is a ligand-analog having at least one common epitope with a ligand so as to be specifically recognizable by a common antibody;
- R.sub.2 is hydrogen or a group represented by R.sub.1 ;
- and
- R.sub.3 is hydrogen, amino or carboxyl;
- and biologically acceptable salts thereof;
- and an antibody capable of specifically recognizing said ligand and said tracer;
- and then determining the amount of tracer bound to antibody by fluorescence polarization techniques as a measure of the amount of ligand in the sample.
4 more elements
Dependencies are inferred from the claim text.