Peptide synthesis reagents and method of use
AssigneeResearch Corporation
InventorsJames P. Tam, William F. Heath, Jr., Robert B. Merrifield
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Abstract
A method of releasing a functional group present in an amino acid or amino acyl residue from a resin or protecting residue which is bonded to the functional group by a linkage having proton affinity, which comprises: reacting the functional group bonded to the organic residue, with a mixture of HF and a base for a time and under conditions effective to produce the release; wherein the amounts of HF and base in the mixture are adjusted so that said release occurs substantially by an S.sub.N 2 mechanism.
Claims19
In a method of synthesizing peptides by the solid phase methodology, wherein the growing peptide chain is covalently attached by a functional group through a linkage having proton affinity, to the organic residue of a resin, the improvement wherein said peptide is detached from said resin using a mixture of HF and a base selected from the group consisting of di-C.sub.1 -C.sub.1O -alkyl sulfides and alkyl phenyl sulfides, wherein the amounts of HF and base in said mixture are adjusted so that said detachment occurs predominantly by an S.sub.N 2 mechanism, said HF being present in said mixture in an amount between 0.1 and 60% by volume.
Dependencies are inferred from the claim text.