Vermiculite as a carrier support for immobilized biological materials
AssigneeGenex Corporation
InventorWayne E. Swann
Life of this patent
Estimating the term…
Abstract
Biological materials are immobilized by being absorbed into vermiculite particles which then are coated with a polymeric coating material. A variety of cross-linking, condensing, and gelling agents may be used to strengthen and crosslink the polymer.
Claims36
A method for immobilizing biological materials by preparing an insolubilized biological material composite comprising the steps of:
- (a) adding vermiculite particles to an aqueous medium of biological material;
- (b) allowing said aqueous medium of biological material to absorb into said vermiculite;
- and
- (c) coating said vermiculite with a polymer.
A method for immobilizing biological materials by preparing an insolubilized biological material composite comprising the steps of:
- (a) adding vermiculite particles to an aqueous medium of biological material;
- (b) allowing said aqueous medium of biological material to absorb into said vermiculite;
- (c) coating said vermiculite with a polyalkyleneimine polymer;
- and
- (d) crosslinking said coated vermiculite with an amine cross-linking agent or condensing with a condensing agent.
An insolubilized biological material composite comprising a biologically active material adsorbed into particles of vermiculite which are immobilized within a polymer.
An insolubilized biological material composite comprising a biologically active material adsorbed into particles of vermiculite which are immobilized within a polymer, wherein said polymer is cross-linked with a cross-linking agent.
An insolubilized biological material composite comprising a biologically active material adsorbed into particles of vermiculite which are immobilized within a polymer wherein said polymer is condensed with a condensing agent.
A method for producing aspartic acid comprising contacting, under aspartic acid producing conditions, a substrate containing ammonium fumarate with an insolubilized biological material composite of aspartase or aspartase containing microbial cells absorbed into vermiculite particles and immobilized within a polymer, wherein the immobilized vermiculite is cross-linked with a cross-linking agent or condensed with a condensing agent.
A method for producing tryptophan comprising contacting, under tryptophan producing conditions, a substrate containing indole and serine with an insolubilized biological material composite of tryptophan synthetase or tryptophan synthetase containing microbial cells absorbed into vermiculite particles and immobilized within a polymer, wherein the immobilized vermiculite is cross-linked with a cross-linking agent or condensed with a condensing agent.
A method for producing L-phenylalanine comprising contacting, under L-phenylalanine producing conditions, a substrate containing ammonium cinnamate with an insolubilized biological material composite of phenylalanine ammonia lyase or phenylalanine ammonia lyase-containing microbial cells absorbed into vermiculite particles and immobilized within a polymer.
Dependencies are inferred from the claim text.