Plasmids
AssigneeKyowa Hakko Kogyo Co., Ltd.
InventorsRyoichi Katsumata, Tetsuo Oka, Akira Furuya
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Abstract
Disclosed are novel plasmids which can replicate autonomously in microorganisms belonging to the genus Corynebacterium or Brevibacterium and which carry a gene for resistance to streptomycin and/or spectinomycin. The plasmids are useful as a cloning vector.
Claims9
An isolated plasmid pCG 4 characterized by a molecular weight of about 19 megadaltons, a number of cleavage sites for the restriction endonucleases, Eco RI, Bam HI, Hind III, Pst I and Sal I of 4, 7, 9, 6 and 6 respectively, and carrying a gene for resistance to a least one of streptomycin and spectinomycin.
A biologically pure culture of the microorganism Corynebacterium glutamicum LA 103/pCG 11, ATCC 39022 which carries the plasmid pCG 11.
A biologically pure culture of the microorganism Corynebacterium glutamicum 225-250, having the identifying characteristics of ATCC 31830 and which carries the plasmid pCG 4.
A biologically pure culture of a microorganism selected from the group consisting of Corynebacterium herculis ATCC 13868/pCG 4, Brevibacterium flavum ATCC 14067/pCG 4 and Brevibacterium lactofermentum ATCC13655/pCG 4 having the identifying characteristics of ATCC 31837, 31838 and 31839 respectively and carrying the plasmid pCG 4.
Dependencies are inferred from the claim text.