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US 4,499,052 AGranted
Apparatus for distinguishing multiple subpopulations of cells
AssigneeBecton, Dickinson and Company
InventorMack J. Fulwyler
Life of this patent
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AssigneeBecton, Dickinson and Company
InventorMack J. Fulwyler
Estimating the term…
A method of distinguishing multiple subpopulations of cells from a single sample of cells of a variety of types comprises labeling particles with two or more marking agents. These particles are marked in a plurality of different pre-selected ratios of the agents ranging between zero percent and one hundred percent of each agent. Each such agent has distinguishing, quantifiable marking characteristics. The differently labeled particles are mixed with cells suspected of having specific receptors for the differently labeled particles. Each cell is analyzed to determine the ratio of any two identifiable marking characteristics associated with each cell so that it can be classified in a subpopulation category if its ratio of marking characteristics is related to one of the pre-selected ratios of marking agents. An apparatus for carrying out the above-described method is also within the purview of the present invention.
An apparatus for distinguishing multiple subpopulations of particles, each of which have been labeled with different fluorochromes in pre-determined ratios comprising:
An apparatus for distinguishing multiple subpopulations of particles from a sample of cells flowing in a liquid path, said particles having been labeled in pre-determined ratios with different marking agents having quantifiable characteristics, comprising:
Dependencies are inferred from the claim text.