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US 4,497,900 AGranted
Immunoassay for Neisseria gonorrhoeae antigens
AssigneeAbbott Laboratories
InventorsDinah Abram, Alan S. Armstrong, John E. Herrmann, Chung-Mei Ling
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Abstract
The present disclosure relates to a solid phase immunoassay for the detection of Neisseria gonorrhoeae antigens in a clinical specimen, wherein the Neisseria gonorrhoeae antigens to be determined are coated or adsorbed on the solid phase.
Claims15
A method for determining Neisseria gonorrhoeae antigen in a clinical specimen comprising:
- (a) lysing Neisseria gonorrhoeae cells in the specimen to release Neisseria gonorrhoeae antigen;
- (b) coating a bare solid support with the Neisseria gonorrhoeae antigen;
- (c) separating the antigen coated solid support from the specimen;
- (d) treating the antigen coated solid support with gonococcal antibody to form antigen-gonococcal antibody complex on the solid support;
- (e) separating the antigen-gonococcal antibody complex from unbound gonococcal antibody;
- (f) treating the antigen-gonococcal antibody complex with labeled antiglobulin, to form an antigen-gonococcal antibody-labeled antiglobulin complex on the solid support;
- (g) separating the antigen-gonococcal antibody-labeled antiglobulin complex from unbound labeled antiglobulin;
- and
- (h) determining the labeled antiglobulin bound to the antigen-gonococcal antibody complex as a measure of the Neisseria gonorrhoeae antigen in the specimen.
6 more elements
A method for determining Neisseria gonorrhoeae antigen in a clinical specimen comprising:
- (a) lysing Neisseria gonorrhoeae cells in the specimen to release Neisseria gonorrhoeae antigen;
- (b) coating a bare solid support with the Neisseria gonorrhoeae antigen;
- (c) separating the antigen coated solid support from the specimen;
- (d) treating the antigen coated solid support with gonococcal antibody to form an antigen-gonococcal antibody complex on the solid support;
- (e) separating the antigen-gonococcal antibody complex from unbound gonococcal antibody;
- (f) treating the antigen-gonococcal antibody complex with antiglobulin, to form an antigen-gonococcal antibody-antiglobulin complex on the solid support;
- (g) separating the antigen-gonococcal antibody-antiglobulin complex from unbound antiglobulin;
- (h) treating the antigen-gonococcal antibody-antiglobulin complex with labeled antibody to the antiglobulin to form an antigen-gonococcal antibody-antiglobulin-labeled antibody complex on the solid support;
- (i) separating the antigen-gonococcal antibody-antigloblin-labeled antibody complex from unbound labeled antibody;
- and
- (j) determining the labeled antibody bound to the antigen-gonococcal antibody-antiglobulin complex as a measure of the Neisseria gonorrhoeae antigen in the specimen.
8 more elements
A method for determining Neisseria gonorrhoeae antigen in a clinical speciman comprising:
- (a) lysing Neisseria gonorrhoeae cells in the specimen to release Neisseria gonorrhoeae antigen;
- (b) coating the bare solid support with the Neisseria gonorrhoeae antigen;
- (c) separating the antigen coated solid support from the specimen;
- (d) treating the antigen coated solid support with labeled gonococcal antibody;
- (e) separating the antigen coated solid support treated with labeled gonococcal antibody from unbound labeled gonococcal antibody;
- and
- (f) determining the labeled gonococcal antibody bound to the antigen coated solid support as a measure of the Neisseria gonorrhoeae antigen in the specimen.
4 more elements
Dependencies are inferred from the claim text.