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AssigneeUniversity of Connecticut
InventorJ. Herbert Waite
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A process is provided for purifying and stabilizing marine mussel polyphenolic proteins rich in 3,4-dihydroxyphenylalanine (dopa) and hydroxyproline (hyp) while obtaining high yields thereof. The process includes the steps of providing an acid soluble extract of the dopa-containing proteins, removing the low molecular weight material from the extract and reacting the remaining proteinaceous material with a water soluble borate at a pH of 7.0-9.0 to provide a soluble borate complex of the dopa-containing protein while precipitating impurities. The complex is separated from the precipitate and may be concentrated for storage or treated with an acetic acid solution and either concentrated or lyophilized and stored under an inert atmosphere. The purified proteins exhibit a dopa:protein index of purity ratio of at least about 0.10.
A process for purifying and stabilizing catechol-containing polyphenolic proteins comprising the steps of:
A purified and stabilized polyphenolic proteinaceous material comprising polypeptide proteins rich in 3,4 dihydroxyphenylalanine (dopa) and hydroxyproline (hyp) having a molecular weight in the range of about 110,000-140,000 with a repeated decapeptide having an amino acid sequence wherein dopa is sequenced predominantly at positions five and nine and hyp is sequenced predominantly at positions six and seven;
Dependencies are inferred from the claim text.