Detection of malignant tumor cells
OwnerSamuel Bogochinventor
Life of this patent
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Abstract
Described herein is the production of two products which are distinct species of anti-malignin antibody, and the production of three artificially produced species of cell each of which has the distinguishing characteristic of manufacturing either one or both species of anti-malignin antibody. These anti-malignin products are useful to detect the presence of cancerous or malignant tumor cells. These anti-malignin products attach preferentially to cancerous or malignant tumor cells in cell collections in vitro or in vivo and thus can be detected by any attached visible or other signal emitter. This preferential attachment to malignant tumor cells also makes these products useful for metabolic and therapeutic purposes.
Claims21
A process for detecting the presence of cancerous or malignant tumor cells in a cell collection, said cells comprising a cancer RECOGNIN, comprising applying to said cell collection a specific monoclonal anti-cancer RECOGNIN antibody, which attaches to cancerous cells and can thereby be detected by visible or signal-emitting means attached to said antibody, said cancer RECOGNIN being derived from cancerous tumor tissue or cells, and characterized by forming a single line precipitate with its specific antibody in quantitative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solutions having an acid or neutral pH, and insoluble at alkaline pH, having a spectrophotometric absorption peak wave length of 280 m.mu.and a molecular weight of from about 3,000 to about 25,000, and further characterized by having an amino acid residue composition characterized by high proportions of glutamic and aspartic acids and high ratios of glutamic and aspartic acids to histidine.
A composition comprising monoclonal anti-MALIGNIN antibody-FAST or a purified fraction thereof, whereby said antibody or a purified fraction thereof attaches to cancerous cells, said cells comprising MALIGNIN;
- and can thereby be detected by visible or signal-emitting means attached to said antibody, said MALIGNIN being derived from brain tumor cells, and which forms a single line precipitate with its specific antibody in quantitative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solution having an acid or neutral pH, and insoluble at an alkaline pH, and has a spectrophotometric absorption peak wave length of 280.mu., a molecular weight of about 10,000, and an amino acid composition approximately as follows:
- ______________________________________ APPROXIMATE NO. OF RESIDUES ______________________________________ Aspartic Acid 9 Threonine 5 Serine 5 Glutamic Acid 13 Proline 4 Glycine 6 Alanine 7 Valine 6 1/2 Cysteine 1 Methionine 2 Isoleucine 4 Leucine 8 Tyrosine 3 Phenylalanine 3 Lysine 6 Histidine 2 Arginine 5 89 ______________________________________
- ammonia and the amino acids cysteic, hydroxyproline, norleucine, isodesmosine, lysinonorleucine and gamma-aminobutyric acid being absent in detectable amounts.
A composition comprising monoclonal anit-MALIGNIN antibody-SLOW or a purified fraction thereof, whereby said antibody or a purified fraction thereof attaches to cancerous cells, said cells comprising MALIGNIN;
- and can thereby be detected by visible or signal-emitting means attached to said antibody, said MALIGNIN being derived from brain tumor cells, and which forms a single line precipitate with its specific antibody in quantitative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solution having an acid or neutral pH, and insoluble at an alkaline pH, and has a spectrophotometric absorption peak wave length of 280 m.mu., a molecular weight of about 10,000, and an amino acid composition approximately as follows:
- ______________________________________ APPROXIMATE NO. OF RESIDUES ______________________________________ Aspartic Acid 9 Threonine 5 Serine 5 Glutamic Acid 13 Proline 4 Glycine 6 Alanine 7 Valine 6 1/2 Cysteine 1 Methionine 2 Isoleucine 4 Leucine 8 Tyrosine 3 Phenylalanine 3 Lysine 6 Histidine 2 Arginine 5 89 ______________________________________
- ammonia and the amino acids cysteic, hydroxyproline, norleucine, isodesmosine, lysinonorleucine and gamma-aminobutyric acid being absent in detectable amounts.
A composition comprising monoclonal anti-MALIGNIN antibody-FAST and SLOW or a purified fraction thereof, whereby said antibody is cytotoxic to and kills said cancer cells, said cells comprising MALIGNIN, whereby said antibody or a purified fraction thereof attaches to cancerous cells and can thereby to detected by visible or signal-emitting means attached to said antibody, said MALIGNIN being derived from brain tumor cells, and which forms a single line precipitate with its specific antibody in quantitive precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solution having an acid or neutral pH, and insoluble at a alkaline pH, and has an spectrophotometric absorption peak wave length of 280 m.mu., a molecular weight of about 10,000, and an amino acid composition approximately as follows:
- ______________________________________ APPROXIMATE NO. OF RESIDUES ______________________________________ Aspartic Acid 9 Threonine 5 Serine 5 Glutamic Acid 13 Proline 4 Glycine 6 Alanine 7 Valine 6 1/2 Cysteine 1 Methionine 2 Isoleucine 4 Leucine 8 Tyrosine 3 Phenylalanine 3 Lysine 6 Histidine 2 Arginine 5 89 ______________________________________
- ammonia and the amino acids cysteic, hydroxyproline, norleucine, isodesmosine, lysinonorleucine and gamma-aminobutyric acid being absent in detectable amounts.
A composition comprising monoclonal anti-cancer RECOGNIN antibody whereby said anti-cancer RECOGNIN antibody is produced by the injection into an organism or collection of cells of a purified cancer RECOGNIN said cancer RECOGNIN having the ability to induce or cause the cells of said organism or collection to produce specific anti-cancer RECOGNIN antibody, wherein such anti-cancer RECOGNIN antibody producing cells are treated to make them self-perpetuating and identified by their production of monoclonal anti-cancer RECOGNIN antibody, said cancer RECOGNIN being derived from cancerous tumor tissue or cells, and characterized by forming a single line precipitate with its specific antibody in quantitative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solutions having an acid or neutral pH, and insoluble at alkaline pH, having a spectrophotometric absorption peak wave length of 280 m.mu. and a molecular weight of from about 3,000 to about 25,000, and further characterized by having an amino acid residue composition characterized by high proportions of glutamic and aspartic acids and high ratios of glutamic and aspartic acids to histidine.
Dependencies are inferred from the claim text.