Immunometric assays using monoclonal antibodies
AssigneeHybritech Incorporated
InventorsGary S. David, Howard E. Greene
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Abstract
"Two-site" or "sandwich" immunometric assay techniques for determination of the presence and/or concentration of antigenic substances in fluids using monoclonal antibodies are described and compared to conventional assays using polyclonal antibodies. Also described are inhibition assays using complexes of antigens with a monoclonal antibody.
Claims33
In an immunometric assay process to determine the presence or concentration of an antigenic substance in a fluid sample comprising forming a ternary complex of the antigenic substance, a first antibody and a second antibody bound to the antigen at a difference site than the first antibody by contacting the sample with said first and second antibodies, the improvement comprising employing a measured amount of a monoclonal antibody having an affinity for the antigenic substance of at least about 10.sup.8 liters/mole for each of said first and second antibodies.
Dependencies are inferred from the claim text.