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US 4,483,929 AGranted
Liposomes with glycolipid-linked antibodies
AssigneeLiposome Technology, Inc.
InventorFrank C. Szoka
Life of this patent
Estimating the term…
Abstract
Lipid vesicles, labelled with encapsulated reporter compositions and bound to antibodies comprise a new class of immunoreagent, useful in immunoassays for ligands.
Claims9
An immunoreactive liposome reagent for use in the determination of a chemical compound capable of entering into an immunospecific reaction with a known antibody, said reagent comprising
- a suspension of liposomes containing reporter molecules, and
- a surface array of molecules of such antibody attached to the liposomes through glycolipid molecules anchored in the liposomes and linked to the antibody molecules by a diimidazole coupling agent.
A method of producing an immunoreactive liposome reagent having an array of surface-bound antibody molecules, said method comprising
- providing a glycolipid,
- linking such antibody molecules covalently to molecules of the glycolipid, in an aqueous medium, with a diimidazole coupling agent, and
- mixing the resultant antibody-glycolipid couple with liposomes in an aqueous medium to produce binding of the antibody to the liposome surface through the linked glycolipid.
Dependencies are inferred from the claim text.