Inactivation of enzymes
AssigneeAMF Incorporated
InventorsCharles R. Carpenter, Robert H. Dodge, Kenneth A. Rosanoff
Life of this patent
Estimating the term…
Abstract
A method for inactivating enzymes, particularly enzymes found in human serum. The method comprises reacting the enzyme in a suitable medium, e.g. human serum, with an inactivating amount of peracetic acid. In the preferred embodiment the treated serum is subsequently neutralized and dialyzed. Preferably, the serums are used as standards for calibration or as a control in assay kits, e.g. RIA, and EIA or enzyme kits, for the determination of specific ligands and enzymes.
Claims15
A method of reducing enzyme activity in a serum with a minimum effect on other constituents in the serum comprising contacting the serum with an enzyme inactivating amount of a peracetic acid to produce a treated serum, wherein the inactivating amount is from about 0.2% to about 1% peracetic acid by weight of serum, wherein said serum does not degrade an amount of enzyme sensitive labile peptide mixed therein for at least about 96 hours, and wherein the enzymes are selected from the group consisting of:
- alkaline phosphatase,
- proteases,
- gamma glutamyl transpeptidase (GGT),
- lactate dehydrogenase (LDH),
- creatinin phosphokinase (CPK), and
- lipase.
Dependencies are inferred from the claim text.