Process for the purification of riboflavine-5'-monophosphate
AssigneesMitsubishi Chemical Industries Limited, Wakamoto Pharmaceutical Co., Ltd.
InventorsKenji Yokota, Hiroshi Kusano, Ryuichi Sugimoto
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Abstract
A process for the purification of riboflavine-5'-monophosphate comprising subjecting crude riboflavine-5'-monophosphate to chromatography using a column packed with a weakly basic anion-exchange resin having a skeleton of a crosslinked polyacrylic acid ester or polymethacrylic acid ester in which at least a part of the ester linkages thereof are converted into amide linkages represented by the following formula (1): ##STR1## to obtain a fraction of riboflavine-5'-monophosphate containing a reduced amount of impurities from an eluate of the column is disclosed.
Claims10
A process for the purification of riboflavine-5'-monophosphate comprising subjecting crude riboflavine-5'-monophosphate containing riboflavine-4'-monophosphate to chromatography using a column packed with a weakly basic anion-exchange resin having a skeleton of a crosslinked polyacrylic acid ester or polymethacrylic acid ester in which at least a part of the ester linkages thereof are converted into amide linkages represented by the following formula (I):
- ##STR7## wherein R.sub.1 represents a group denoted by ##STR8## in which n is an integer of from 2 to 6, m is an integer of from 2 to 4, and R.sub.3 and R.sub.4 are each a hydrogen atom or an alkyl group having 1 to 6 carbon atoms;
- and R.sub.2 is the same as defined in R.sub.1 or represents a hydrogen atom or an alkyl group having 1 to 6 carbon atoms, to obtain a fraction of riboflavine-5'-monophosphate containing a reduced amount of riboflavine-4'-monophosphate from an eluate of the column.
Dependencies are inferred from the claim text.