Recombinant monoclonal antibodies
AssigneeThe University of Texas System Cancer Center
InventorChristopher L. Reading
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Abstract
Antibodies having binding affinity for two desired antigens, hereinafter "recombinant monoclonal antibodies"; recombinant monoclonal antibodies produced by a quadroma cell or a trioma cell; and methods for producing recombinant monoclonal antibodies by means of a quadroma cell or a trioma cell, wherein a quadroma cell is the fusion product of a hybridoma cell which produces an antibody having specific binding affinity to one desired antigen and a hybridoma cell which produces an antibody having specific binding affinity for another desired antigen, and wherein a trioma cell is the fusion product of a hybridoma cell which produces an antibody having specific binding affinity to one desired antigen and a lymphocyte which produces an antibody having specific binding affinity to another desired antigen.
Claims50
An antibody with binding affinity for two different desired antigens.
An antibody produced by a quadroma cell, wherein said antibody has specific binding affinity for two desired antigens and wherein said quadroma cell comprises the fusion product of:
- (a) a hyridoma cell which produces an antibody having specific binding affinity to one desired antigen, and
- (b) a hybridoma cell which produces an antibody having specific binding affinity for another desired antigen.
An antibody produced by a trioma cell, wherein said antibody has specific binding affinity for two desired antigens and wherein said trioma cell comprises the fusion product of:
- (a) a hybridoma cell which produces an antibody having specific binding affinity to one desired antigen, and
- (b) a lymphocyte which produces an antibody having specific binding affinity to another desired antigen.
A method of producing a recombinant monoclonal antibody comprising incubating a quadroma cell in culture or in the peritoneal cavity of a mouse, and separating soluble protein from the culture supernatant or ascites fluid, respectively.
A method of producing a recombinant monoclonal antibody comprising incubating a trioma cell in culture or in the peritoneal cavity of a mouse, and separating soluble protein from the culture supernatant or ascites fluid, respectively.
Dependencies are inferred from the claim text.