Rat myeloma cell lines
AssigneeNational Research Development Corporation
InventorsCesar Milstein, Bruce W. Wright
Life of this patent
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Abstract
A rat myeloma cell line which does not express an immunoglobulin chain, YB2/3.0.Ag.20, is prepared from the cell line Y3-Ag 1.2.3 via a hybrid myeloma cell line. This cell line, and variants thereof prepared by passaging and/or cloning the line, may be fused with immunocyte cells from an animal sensitized to an immunogen to produce hybrid myeloma cell lines which provide a source of monoclonal antibodies to said immunogen.
Claims23
The rat myeloma cell line YB2/3.0.Ag.20.
A rat myeloma cell line being a variant of the cell line YB2/3.0.Ag.20 prepared by passaging and/or cloning cell line YB2/3.0.Ag.20.
A hybrid myeloma cell line, prepared through the fusion of YB2/3.0.Ag.20 or a variant thereof prepared by passaging and/or cloning YB2/3.0.Ag.20, with immunocyte cells from an animal sensitized to an antigen.
A process for producing a rat myeloma cell line which comprises passaging and/or cloning YB2/3.0.Ag.20.
An antibody production process which comprises preparing a hybrid myeloma cell line by the fusion of cells of the cell line YB2/3.0.Ag.20 with immunocyte cells sensitized to an immunogen and culturing the hybrid in an in vitro or in vivo culture medium therefor to produce antibodies against the sensitizing immunogen.
Dependencies are inferred from the claim text.