Purified antineoplaston fractions and methods of treating neoplastic disease

A process for extracting from urine a peptide fraction containing the compound 3-[N-phenylacetylaminopiperidine]-2, 6-dion comprising the steps of:

• providing a volume of urine;
• separating from the urine particulate matter and matter having a molecular weight greater than about 5000;
• acidifying the urine;
• separating from the acidified urine any precipitated matter;
• introducing the acidified urine to a first chromatography column containing a polymeric resin adsorbent;
• eluting the column to recover an eluate;
• adjusting the pH of the eluate to between about 2 and 8;
• introducing the adjusted eluate to a second chromatography column;
• and
• recovering the cytostatic active peptide fraction, said peptide fraction containing the compound 3-[N-phenylacetylaminopiperidine]-2, 6-dion.

A process for extracting from urine a peptide fraction containing the compound 3-[N-phenylacetylaminopiperidine]-2, 6-dion comprising the steps of:

• providing a volume of urine;
• separating from the urine particulate matter and matter having a molecular weight greater than about 5000;
• acidifying the urine;
• separating from the acidified urine any precipitated matter;
• introducing the acidified urine to a first chromatography column containing a resin adsorbent;
• eluting the column with an alkaline wash;
• collecting the alkaline wash eluate;
• adjusting the pH of the alkaline wash eluate from about pH 2 to about 8;
• oxidizing the acidified eluate;
• introducing the oxidized acidified eluate to a second chromatography column;
• and
• recovering the cytostatic active peptide fraction, said peptide fraction containing the compound 3-[N-phenylacetylaminopiperidine]-2, 6-dion.

A process for extracting from urine a peptide fraction containing the compound 3-[N-phenylacetylaminopiperidine]-2, 6-dion comprising the steps of:

• providing a volume of urine;
• subjecting the urine to oxidation;
• adjusting the pH of the urine to between about 2 and about 8;
• removing from the oxidized urine any precipitated matter;
• introducing the oxidized urine to a chromatography column;
• and
• recovering the cytostatic active peptide fraction, said peptide fraction containing the compound 3-[N-phenylacetylaminopiperidine]-2, 6-dion.

A process for extracting from urine a peptide fraction containing the compound 3-[N-phenylacetylaminopiperidine]-2, 6-dione comprising the steps of:

• providing a volume of urine;
• separating from the urine particulate matter and matter having a molecular weight greater than about 5000;
• acidifying the urine;
• separating from the acidified urine any precipitated matter;
• introducing the acidified urine to a chromatography column;
• eluting the column with a lower alkyl alcohol having from 1 to 8 carbon atoms;
• and
• recovering the cytostatic active peptide fraction, said peptide fraction containing the compound 3-[N-phenylacetylaminopiperidine]-2, 6-dion.