Lectin-containing anti-viral vaccines for domestic animals and method of preparation
AssigneeIowa State University Research Foundation, Inc.
InventorsPatricia M. Gough, Kenneth B. Platt
Life of this patent
Estimating the term…
Abstract
Anti-viral vaccines for domestic animals are prepared from glycoprotein envelope whole virus or antigenic glycoprotein obtained therefrom by complexing the glycoprotein antigenic agent with a lectin, which is preferably a mitogenic lectin. The recovery of the immunizing agent from an aqueous solution or suspension is facilitated since the lectin-glycoprotein complex is readily recoverable. The mitogenic lectin administered in the vaccine with the glycoprotein antigen can enhance the anamnestic response, in effect, acting as an adjuvant.
Claims17
A veterinary vaccine in parenterally injectable dose form, comprising an effective immunizing dose amount of viral antigen immunizing agent selected from the class consisting of (i) whole viruses having envelopes containing at least one glycoprotein or (ii) the separated viral envelope glycoprotein obtained from such viruses, and a mitogenic lectin complexed with the glycoprotein of said immunizing agent, said complexed lectin providing binding sites available for lymphocytes, and said vaccine containing at least from 0.01 to 0.1 milligrams (mg) of said mitogenic lectin per milligram (mg) of glycoprotein.
The method of preparing and administering a veterinary vaccine, comprising:
- (a) preparing an aqueous solution or suspension of a viral antigen immunizing agent selected from the class consisting of (i) whole viruses having envelopes containing at least one glycoprotein or (ii) the separated viral envelope glycoprotein obtained from such viruses;
- (b) contacting said immunizing agent in said aqueous solution or suspension with a lectin reagent capable of complexing with the glycoprotein of said immunizing agent, said lectin reagent being a mitogenic lectin and a sufficient amount of said lectin reagent being employed to form a readily separable complex from said immunizing agent;
- (c) recovering the thus-formed lectin-glycoprotein complex of the immunizing agent;
- (d) preparing a parenterally injectable vaccine from said immunizing agent without separating the lectin therefrom, the lectin in said complex having binding sites available for lymphocytes;
- and
- (e) administering an effective immunizing dose amount of said vaccine by parenteral injection to an animal capable of being immunized by said immunizing agent.
A veterinary vaccine in parenterally injectable dose form, comprising an effective immunizing dose amount of viral antigen immunizing agent consisting of viral envelope glycoprotein separated from a virus having an envelope containing at least one glycoprotein, and a mitogenic lectin complexed with said immunizing agent, said complexed lectin providing binding sites available for lymphocytes, and said vaccine containing at least from 0.01 to 0.1 milligrams (mg) of said mitogenic lectin per milligram (mg) of glycoprotein.
The method of preparing and administering a veterinary vaccine comprising:
- (a) preparing an aqueous solution of a viral antigen immunizing agent consisting of separated viral envelope glycoprotein obtained from a virus having an envelope containing at least one glycoprotein;
- (b) contacting said immunizing agent in said aqueous solution with a lectin reagent capable of complexing with the glycoprotein of said immunizing agent, said lectin reagent being a mitogenic lectin and a sufficient amount of said lectin reagent being employed to form a readily separable complex from said immunizing agent;
- (c) recovering the thus-formed lectin-glycoprotein complex;
- (d) preparing a parenterally injectable vaccine from said immunizing agent without separating the lectin therefrom the lectin in said complex having binding sites available for lymphocytes;
- and
- (e) administering an effective immunizing dose amount of said vaccine by parenteral injecting to an animal capable of being immunized by said immunizing agent.
Dependencies are inferred from the claim text.