Digoxigenin immunogens, antibodies, labeled conjugates, and related derivatives
AssigneeMiles Laboratories, Inc.
InventorJames P. Albarella
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Abstract
Immunogen conjugates comprising N-substituted-amino-3-desoxydigoxigenin derivatives coupled to conventional immunogenic carrier materials, and antibodies raised against such conjugates. Also provided are labeled digoxigenin conjugates for use with the digoxigenin antibodies in preferred immunoassay techniques for determining digoxin in biological fluids.
Claims22
A digoxigenin immunogen conjugate of the formula:
- ##STR6## wherein Carrier is an immunogenic carrier material, R is a bond or a linking group, one of l and m is one and the other is zero, n is an integer from 2 through 10, and p is on the average from 1 to about 50.
A digoxigenin immunogen conjugate of the formula:
- ##STR7## wherein Carrier is an immunogenic protein or polypeptide Carrier material, Y is an amide group linking carboxyl or amino groups on the carrier material to the digoxigenin moiety, n is an integer from 2 through 10, and p is on the average from 1 to the number of available amide coupling sites on the carrier material.
A digoxigenin derivative of the formula:
- ##STR8## wherein n is an integer from 2 through 10.
A .beta.-galactosyl-umbelliferone-digoxigenin conjugate of the formula:
- ##STR9## and n is an integer from 2 through 10.
A flavin adenine dinucleotide-digoxigenin conjugate of the formula:
- ##STR10## wherein Ribose-(Phos).sub.2 -Riboflavin represents the riboflavin-pyrophosphate-ribose residue in flavin adenine dinucleotide, r is an integer from 2 through 10, and n is an integer from 2 through 10.
Dependencies are inferred from the claim text.