Simple and rapid method for extraction of proteins from bacteria
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsSaumya Bhaduri, Paul H. Demchick
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Abstract
44A simple, rapid method for extracting proteins from pathogenic and nonpathogenic bacteria is described.
Claims3
A method of extracting proteins from bacteria comprising treating bacterial cells with a suitable volatile organic solvent for about five minutes, centrifuging the treated cells and the solvent to collect the cells, removing the volatile residues from the collected cells with a stream of inert gas, and extracting the proteins from the cells with an aqueous phase solvent.
A method of extracting proteins from bacteria comprising suspending the bacterial cells in ice-cold acetone for about five minutes, centrifuging the suspension to collect the cells, removing residual acetone with a stream of nitrogen, and extracting the protein with sodium dodecyl sulfate.
Dependencies are inferred from the claim text.