Back to results
US 4,461,760 AGranted
Anticancer drugs
AssigneeNoda Shokukin Kogyo Co., Ltd.
InventorsNobuhiko Sugano, Chiyokichi Iizuka, Hiroaki Maeda
Life of this patent
Estimating the term…
Abstract
Anticancer drugs consisting of sugar and protein composed primarily of xylose which are obtained from the nutrient medium and tissue-medium of Basidiomycetes such as Lentinus edodes which has been cultured in a solid medium rich is xylose such as bagasse is described.
Claims5
A composition for treatment of cancer by intraperitoneal or oral administration, consisting of sugar and protein extract, wherein the principal sugar is xylose, obtained from the nutrient- and tissue-medium of Lentinous edodes, the method comprising the steps of:
- (A) culturing the mycelia of Lentinus edodes in a solid medium primarily composed of a xylose-rich bagasse,
- (B) crushing the mixture of nutrient- and tissue-medium of the mycelia of Lentinus edodes and, after adding 5 liters of water per 800 grams of crushed material at a pH of 3 to 8, blending and stirring the nutrient medium and tissue medium at a temperature of from 40.degree. to 60.degree. C.,
- (C) compressing, filtering and sterilizing the suspension obtained from Step B,
- (D) freeze-drying the filtrate from Step C to form a powder called LEM,
- (E) dissolving the LEM powder in water and then precipitating a powder, using a four-fold volume of ethyl alcohol, washing the precipitate with an 80% ethyl alcohol solution, and then centrifuging and freeze-drying the resulting material to obtain a powder, called LAP, and
- (F) fractionating the LAP obtained from Step E by column chromatography on Sephalose to obtain LAP-1 and LAP-2.
3 more elements
Dependencies are inferred from the claim text.