Babesiosis vaccine prepared from soluble parasitic antigen factors isolated from disintegration of Babesia infected erythrocites
OwnerBrian V. Goodger, David F. Mahoney +1inventors
InventorsBrian V. Goodger, David F. Mahoney, Ian G. Wright
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Abstract
This invention relates to a method of preparation of a babesiosis vaccine which involves treating a suspension of Babesia infected erythrocytes. The erythrocytes are disintegrated, separated into soluble and insoluble fractions and subsequently fibrinogen and contaminated Babesia antigen is removed from the soluble fraction. The residual soluble fraction is then combined with an adjuvant to form the babesiosis vaccine. The invention also includes within its scope a vaccine derived from the abovementioned method and a method of administration of the vaccine to cattle.
Claims42
A method of preparation of a babesiosis vaccine, including the steps of:
- (i) disintegrating a suspension of Babesia bovis infected erythrocytes;
- (ii) separating the soluble and insoluble fractions contained in the disintegrated erythrocyte suspension;
- (iii) removing fibrinogen and contaminated babesial antigen from the soluble fraction of the disintegrated erythrocyte suspension;
- and
- (iv) combining the soluble fraction after step
- (iii) with an adjuvant to form the babesiosis vaccine.
A vaccine for administration to cattle including:
- a soluble fraction derived from a disintegrated suspension of Babesia bovis infected erythrocytes and having fibrinogen and contaminated babesial antigen removed therefrom;
- and an adjuvant.
A method of preparation of a babesiosis vaccine including the steps of;
- (i) disintegrating a suspension of erythrocytes infect with the parasite Babesia bovis so as to substantially solubilise parasite material contained therein;
- (ii) separating from the resulting mixture a soluble fraction containing essentially parasite material substantially free or erythrocytes;
- (iii) removing fibrinogen and contaminated babesial antigen from said soluble fraction;
- and
- (iv) combining said soluble fraction with an adjuvant.
A vaccine for administration to cattle including a soluble fraction derived from a disintegrated suspension of erythrocytes infected by the parasite Babesia bovis containing parasite material substantially free of erythrocyte material and having fibrinogen and contaminated babesial antigen removed therefrom, and an adjuvant.
Dependencies are inferred from the claim text.