Method of the preparation of improved mutant strain of Bordetella bronchiseptica useful as live attenuated vaccine for prophlaxis of B. bronchiseptica infection
AssigneesJuridical Foundation The Chemo-Sero-Therapeutic Research Institute, Nippon Vaccine Co., Ltd., Takeshi Shimizu
InventorTakeshi Shimizu
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Abstract
A method for the preparation of a new mutant strain of Bordetella bronchiseptica having a plural of hereditary chromosomal markers of at least temperature-sensitivity and urease negative, more particularly a phase I mutant strain of B. bronchiseptica having excellent immunity and high safety, which is useful for the preparation of a live attenuated vaccine for prophylaxis of B. bronchiseptica infection, and also a live attenuated vaccine prepared therefrom, and use thereof.
Claims10
A method for the preparation of a strain of Bordetella bronchiseptica having hereditary chromosomal markers of being temperature-sensitive and urease negative and which is useful in the production of a live vaccine for prophylaxis of Bordetella bronchiseptica infection, which comprises subjecting a strain of Bordetella bronchiseptica to a conventional means for mutating microorganisms to obtain a strain having the hereditary markers of being temperature-sensitive in that it can grow at 32.degree. C. but not above 34.degree. C. and urease negative, culturing the strain on a blood agar medium, picking up a cell grown with a comparatively small colony, culturing the cell on a blood agar medium, and repeating the procedure of picking up a cell and culture thereof to convert it into phase I organisms which has completely capsular antigen and is hemolytic.
A method for the prophylaxis of Bordetella bronchiseptica infection, which comprises administering to an animal an effective amount of a live attenuated vaccine prepared with a phase I strain ts-S34.u.sup.- of Bordetella bronchiseptica having hereditary chromosomal markers of being temperature-sensitive in that it can grow at 32.degree. C. but not above 34.degree. C. and urease negative.
A live attenuated vaccine for prophylaxis of Bordetella bronchiseptica infection, which comprises phase I Bordetalla bronchiseptica strain ts-S34.u.sup.- having hereditary chromosomal markers of being temperature-sensitive in that it can grow at 32.degree. C. but not above 34.degree. C. and urease negative, and a carrier therefor.
A biologically pure culture of Bordetella bronchiseptica strain ts-S34.u.sup.- having the hereditary chromosomal markers of being temperature-sensitive in that it can grow at 32.degree. C. but not above 34.degree. C. and urease negative.
Dependencies are inferred from the claim text.