Pseudomonas compositions
AssigneeMicrolife Genetics, Inc.
InventorsRonald H. Olsen, Peter A. Vandenbergh
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Abstract
Compositions of selected strains of Pseudomonas bacteria having the ability to utilize halogenated aromatic compounds as a sole carbon source are described. The bacteria are isolated from environments where they have been in long association with halogenated aromatic compounds, usually analagous compounds. First L-tryptophan and then a halogenated aromatic hydrocarbon are used as sole carbon sources for isolating and testing the selected strains. The isolated Pseudomonas strains are Pseudomonas putida; Pseudomonas sp. NRRL-B-12,538 or NRRL-B-12,539 or transfer derivatives thereof and are useful for degrading halogenated aromatic pollutants, particularly mono- and di-chloroaromatics.
Claims19
A biologically pure culture of a bacterium of the genus Pseudomonas in a containing genetic material which promotes utilization of a wide variety of halogenated hydrocarbons as sole carbon sources and wherein the strain grows faster in the presence of o-, m- or p-chlorotoluene than toluene as a sole carbon source, the genetic material being isolatable from a strain of Pseudomonas putida or Pseudomonas sp. NRRL-B-12,538 or NRRL-B-12,539 in contact for extended periods of time with halogenated aromatic compounds first by growth in a minimal medium including L-tryptophan as a sole carbon source and then growth in a second minimal medium in the presence of a halogenated aromatic compound as a second sole carbon source and wherein growth of the bacterium is determined using a minimal medium agar plate in the presence of vapors of toluene or o-, m- or p-chlorotoluene as a sole carbon source at 25.degree. to 30.degree. C.
A biologically pure culture of a bacterium of the genus Pseudomonas in a having genetic material from Pseudomonas putida isolated by the method of:
- (a) providing a sample from an environment which contains halogenated aromatic compounds which have been in contact with bacteria of the genus Pseudomonas including Pseudomonas putida for extended periods of time;
- (b) isolating the Pseudomonas from the sample in a minimal medium containing L-tryptophan as the sole carbon source along with a nitrogen source and essential minerals;
- and
- (c) re-isolating the L-tryptophan isolated strains of the Pseudomonas in the confined space on a minimal medium containing as a sole carbon source a halogenated aromatic compound wherein the bacterium grows faster in the presence of o, p, or m-chlorotoluene than toluene as a sole carbon source and wherein growth of the bacterium is determined using a minimal medium agar plate in the presence of vapors of toluene or o-, m- or p-chlorotoluene as a sole carbon source at 25.degree. to 30.degree. C.
A biologically pure culture of a bacterium of the genus Pseudomonas containing genetic material which encodes for halogenated aromatic compound degradation and in particular grows faster in the presence of o-, m- or p-chlorotoluene than toluene as a sole carbon source and which is derived from a Pseudomonas isolated from soil, wherein the isolated Pseudomonas has been in contact with a halogenated hydrocarbon lubricant in the soil for extended periods of time adjacent to an oil well and wherein growth of the bacterium is determined using a minimal medium agar plate in the presence of vapors of toluene or o-, m- or p-chlorotoluene as a sole carbon source at 25.degree. to 30.degree. C.
A biologically pure culture of a bacterium which is Pseudomonas putida NRRL-B-12,536.
A biologically pure culture of a bacterium which is Pseudomonas sp. NRRL-B-12,538.
A biologically pure culture of a bacterium which is Pseudomonas sp. NRRL-B-12,539.
A biologically pure culture of a bacterium which is Pseudomonas aeruginosa NRRL-B-12,536.
Dependencies are inferred from the claim text.