Bacillus thuringiensis crystal protein in Escherichia coli
AssigneeThe Board of Regents of the University of Washington
InventorsH. Ernest Schnepf, Helen R. Whiteley
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Abstract
Expression of the crystal protein of Bacillus thuringiensis in E. coli is described by use of plasmids containing heterologous DNA coding for the crystal protein. Genetically engineered bacterial host strains transformed by the recombinant plasmids of the invention express B. thuringiensis crystal proteins.
Claims6
An Excherichia coli bacterial strain transformed to express a polypeptide of 130,000 Mr having the immunological properties of crystal protein of Bacillus thuringiensis.
A hybrid recombinant plasmid capable of replication in an Escherichia coli bacterial host species, said plasmid containing expressible heterologous DNA coding for a polypeptide of 130,000 Mr which has the immunological properties of crystal protein of Bacillus thuringiensis, said plasmid further including an expression mechanism for said heterologous DNA which is recognized by the host species' system.
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