Test method for the laboratory diagnosis of Gonorrhea and test strain of neisseria gonorrhoeae

The method for the laboratory diagnosis of gonorrhea by detecting DNA of Neisseria gonorrhoeae in a patient's specimen material, comprising the steps of (1) applying a non-toxic preparation of a patient's specimen material, directly to a culture of Neisseria gonorrhoeae ATCC 31953, which has abnormal growth characteristics, which is in or on a biological medium suitable for growth of normal Neisseria gonorrhoeae, and (2) observing for the restoration of normal growth to the abnormal growth strain Neisseria gonorrhoeae ATCC 31953, in or on the biological medium of step (1), under conditions normal for growth of Neisseria gonorrhoeae.

A diagnostic test kit useful in the diagnosis of gonorrhea by detecting DNA of Neisseria gonorrhoeae in a patient's specimen material, the kit comprising:

• (1) a container with an aliquot of a test strain of Neisseria gonorrhoeae ATCC 31953 which microorganism is abnormal in that it has characteristically poor growth on common chocolate agar at a temperature within the range of from about 30.degree. C. to about 37.degree. C. in a CO.sub.2 atmosphere suitable for growth of normal Neisseria gonorrhoeae,
• (2) a container with a solid or liquid medium capable of supporting growth of the test strain, as well as normal Neisseria gonorrhoeae, whereby, said aliquot can be applied to the solid or liquid medium and thereon exposed to a preparation of a patient's specimen material which is non-toxic to said abnormal test strain;
• the development of a normal growth of Neisseria gonorrhoeae colonies indicating the presence of Neisseria gonorrhoeae DNA in the patient's specimen material.