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US 4,446,122 AGranted
Purified human prostate antigen
AssigneeResearch Corporation
InventorsTsann M. Chu, Ming C. Wang, Lawrence Papsidero
Life of this patent
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AssigneeResearch Corporation
InventorsTsann M. Chu, Ming C. Wang, Lawrence Papsidero
Estimating the term…
A prostate antigen distinct from prostatic acid phosphatase has been detected in normal, benign hypertrophic and malignant prostatic tissues, but not in other human tissues. The prostate antigen was purified to homogeneity from prostatic tissues by ammonium sulfate precipitation, DEAE-BioGel A anion exchange chromatography, molecular sievings on Sephadex G-100 and Sephadex G-75, and preparative polyacrylamide gel electrophoresis. The purified prostate antigen shows a single protein band on analytical polyacrylamide gel electrophoresis and isoelectric focusing. The molecular weight of purified antigen was estimated by Sephadex G-75 gel filtration to be 33,000 and by sodium dodecyl sulfate polyacrylamide gel electrophoresis to be 34,000 with no subunit. The prostate antigen had an isoelectric point of 6.9.
An in vivo composition of matter comprising an immunochemically effective concentration and amount of a purified human prostate specific antigen occurring in normal and cancerous prostatic tissue and purified to show a single protein band on analytical polyacrylamide gel electrophoresis and isoelectric focusing, said antigen having a molecular weight as determined by Sephadex G-75 gel of filtration of about 33,000 and by a molecular weight as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis of about 34,000 with no subunit and having an isoelectric point pI of 6.9, said antigen further being insoluble in perchloric acid and substantially free of normal serum protein components, water-insoluble cellular material and of prostatic acid phosphatases.
An in vitro composition of matter comprising an immunochemically effective concentration and amount of antibodies against a human prostate specific antigen occurring in normal and cancerous prostatic tissue and purified to show a single protein band on analytical polyacrylamide gel electrophoresis and isoelectric focusing, said antigen having a molecular weight by Sephadex G-75 gel filtration of about 33,000 and by sodium dodecyl sulfate polyacrylamide gel electrophoresis of about 34,000 with no subunit and having an isoelectric point pI of 6.9, said antigen further being insoluble in perchloric acid and said composition being free of antibodies against human prostatic acid phosphatases.
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