Process for making human antibody producing B-lymphocytes
AssigneeSloan-Kettering Institute
InventorMichael K. Hoffmann
Life of this patent
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Abstract
Antibody producing human B-lymphocytes are produced by a process which comprises culturing human B-lymphocytes in a tissue culture medium in the presence of an antigen; helper signal producing agents comprising monocytes or monocyte conditioned medium containing Interleukin 1 (I1-1), and helper T-lymphocytes or helper T-lymphocyte replacing factor; and human serum; and thereafter recovering the antibody producing cells from the medium.
Claims29
Process for making antibody producing human B-lymphocytes which comprises culturing a human lymphoid cell suspension containing human B-lymphocytes and a cell concentration of about 1.times.10.sup.6 to 5.times.10.sup.6 cells/ml in a tissue culture medium containing:
- (a) 0.3 to 0.003% antigen;
- (b) helper signal producing agents consisting essentially of:
- (i) monocytes or conditioned medium containing interleukin 1 derived from said monocytes in an amount to result in a concentration of 0.1 to 30% and
- (ii) helper T-lymphocytes or helper T-lymphocyte replacing factor obtained from activated T-cells;
- and
- (c) about 1-10% human serum;
- and thereafter recovering the antibody producing human B-Lymphocytes from the medium.
Dependencies are inferred from the claim text.