Production of monoclonal antibodies against bacterial adhesins
AssigneeMolecular Genetics, Inc.
InventorPeter L. Sadowski
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Abstract
Monoclonal antibodies specific for surface antigens of bacteria which act as adhesins between prokaryotic and eukaryotic cells and, in particular, K-99 pili, and methods for production thereof are described. These monoclonal antibodies may be used for the prophylactic and therapeutic treatment of diseases induced by adhesin-bearing pathogens in animals and humans, and for the diagnostic identification of adhesin-bearing bacteria.
Claims43
A process for producing monoclonal antibodies against an adhesion of a pathogenic bacterium, which adhesin mediates attachment of said bacterium to mucocutaneous tissue, comprising:
- propagating a hybridoma formed by fusing a cell capable of producing antibodies against an adhesin of a pathogenic bacterium, which adhesin mediates attachment of said bacterium to mucocutaneous tissue, with a myeloma cell and harvesting the antibodies produced by said hybridoma.
A process for producing monoclonal antibodies against an adhesin of a pathogenic bacterium, which adhesin mediates attachment of said bacterium to mucocutaneous tissue, comprising:
- (a) propagating a hybridoma (i) formed by fusing a P3/NSI/1-Ag4-1 myeloma cell with a murine cell capable of producing antibodies against an adhesin of a pathogenic bacterium, which adhesin mediates attachment of said bacterium to mucocutaneous tissue, said murine cell having been obtained from a BALB/c mouse immunized with a bacterial adhesion and (ii) isolated after in vitro culturing in selective HAT medium;
- and (b) harvesting the antibodies produced by said hybridoma.
A continuous cell line which produces monoclonal antibodies against an adhesin of a pathogenic bacterium, which adhesin mediates attachment of said bacterium to mucocutaneous tissue, comprising a hybridoma formed by fusing a cell capable of producing antibodies against an adhesin of a pathogenic bacterium, which adhesin mediates attachment of said bacterium to mucocutaneous tissue, with a myeloma cell.
Hybridoma cell line 2BD4E4, ATCC Number HB8178, formed by fusing a P3/NSI/1-Ag 4-1 murine myeloma cell with a murine spleen cell obtained from a BALB/c mouse immunized with K-99 pili, which hybridoma cell line produces monoclonal antibodies against a K-99 pilus.
Monoclonal antibodies against an adhesin of a pathogenic bacterium, which adhesin mediates attachment of said bacterium to mucocutaneous tissue.
A pharmaceutical composition comprising monoclonal antibodies against an adhesin of a pathogenic bacterium, which adhesin mediates attachment of said bacterium to mucocutaneous tissue, and a pharmaceutical carrier therefor.
A process for producing monoclonal antibodies against an adhesin of a pathogenic bacterium, which adhesin mediates attachment of said bacterium to mucocutaneous tissue, comprising:
- propagating a hybridoma formed by fusing a cell capable of producing antibodies against an adhesin of a pathogenic bacterium, which adhesin mediates attachment of said bacterium to mucocutaneous tissue, the pathogenicity of said bacterium being dependent on noninvasive, adhesin-mediated attachment to and subsequent proliferation on the surface of mucocutaneous tissue, with a myeloma cell and harvesting the antibodies produced by said hybridoma.
Monoclonal antibodies against an adhesin of a pathogenic bacterium, which adhesin mediates attachment of said bacterium to mucocutaneous tissue, the pathogenicity of said bacterium being dependent on noninvasive, adhesin-mediated attachment to and subsequent proliferation on the surface of mucocutaneous tissue.
A continuous cell line which produces monoclonal antibodies against an adhesin of a pathogenic bacterium, which adhesin mediates attachment of said bacterium to mucocutaneous tissue, comprising a hybridoma formed by fusing a cell capable of producing antibodies against an adhesin of a pathogenic bacterium, which adhesin mediates the attachment of said bacterium to mucocutaneous tissue, the pathogenicity of said bacterium being dependent on noninvasive, adhesin-mediated attachment to and subsequent proliferation on the surface of mucocutaneous tissue, with a myeloma cell.
Dependencies are inferred from the claim text.