Unique T-lymphocyte line and products derived therefrom
AssigneeThe Regents of the University of California
InventorsDavid W. Golde, Shirley G. Quan
Life of this patent
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Abstract
Human T-lymphoblast cell line, Proteinaceous products produced therefrom, messenger RNA and DNA expressing the proteinaceous products. A human T-lymphoblast cell line (Mo) maintained as a continuous culture constitutively produces proteins, including immune interferon, neutrophil migration inhibition factor, granulocyte-macrophage colony-stimulating activity and erythroid-potentiating activity, as well as other proteins produced by T-cells.
Claims22
A method for producing in isolatable amounts an excretory protein produced by a T-lymphocyte,
- said method comprising:
- cultivating as a single cell suspension the Mo cell line in a nutrient medium, whereby said excretory proteins are produced and excreted into said nutrient medium.
A method for cloning DNA including a gene capable of expressing a protein product produced by a T-lymphocyte,
- said method comprising:
- isolating messenger RNA from Mo cells;
- separating the messenger RNA coding for expression for a predetermined polypeptide from other messenger RNA;
- generating cDNA with reverse transcriptase from said messenger RNA;
- preparing double-stranded DNA from said cDNA employing DNA polymerase;
- inserting said double-stranded DNA into a vector;
- and
- transforming a host with said vector, whereby said double-stranded DNA is cloned.
A protein composition comprising erythroid potentiating factor in an amount of at least about 50,000 units/mg.
A protein composition comprising colony stimulating factor in an amount sufficient to provide at least about 10 units/mg.
A single cell suspension of the Mo cell line in a nutrient medium.
A genetic library comprising DNA fragments derived by restriction cleavage of DNA from the Mo cell line.
Dependencies are inferred from the claim text.