Method and kit for silver staining substances supported in matrix
AssigneeThe Upjohn Company
InventorsLonnie D. Adams, David W. Sammons
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Abstract
A silver stain procedure wherein a substance capable of binding silver is treated with an aqueous silver salt solution, a reducing solution and an aqueous carbonate or sulfate salt solution and kit useful in practicing same.
Claims15
A method for silver staining a substance supported in a matrix, comprising:
- (a) equilibrating said substance in an aqueous silver salt solution;
- (b) rinsing said substance in water;
- (c) subjecting said substance to a reducing solution;
- and
- (d) immersing said substance in a first, then a second aqueous carbonate or aqueous sulfate salt solution.
A method for silver staining a protein, polypeptide or protein containing substance which is supported in a matrix which comprising equilibrating said substance for about two hours in an aqueous silver nitrate solution containing from about 0.5 to 6 grams of silver salt per liter of water, briefly washing said substance in water, then subjecting said substance for about 5 to 15 minutes to a reducing solution comprising about 1 to 5 ml of 37% formalin followed by immersing said substance in a first 0.075 to 1.5% aqueous sodium carbonate solution for about one hour, then a second 0.15 to 3% aqueous carbonate solution for a total of about six hours.
A mercantile kit comprising multiple containers wherein is contained separately the following reagents:
- a silver salt, a base solution, an aldehyde solution and a carbonate or sulfate salt.
Dependencies are inferred from the claim text.