Strong anion exchange composition and methods
AssigneeVarian Associates, Inc.
InventorSeth R. Abbott
Life of this patent
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Abstract
A strong anion exchange composition useful in monophosphate nucleotide isomer separations is comprised of an inert porous particle having a silyl alkyl ammonium compound fixedly attached by a covalent silicon-oxygen-silicon bond to the surface thereof. A process for synthesizing the strong anion exchange composition in an aqueous solvent is disclosed together with methods for separation of monophosphate nucleotide isomers, highly phophorylated nucleotides and aldopentose carbohydrates using a column packed with this strong anion exchange composition.
Claims29
A method for the separation of components contained in a biological composition, the steps of the method comprising:
- (a) packing a column with a strong anion exchange composition comprising an inert porous particle having a particle size of about 3 to about 20 microns which has covalently attached to the silica surface a silyl alkyl ammonium compound having the general formula:
- ##STR4## wherein R is methyl or ethyl;
- n is an integer ranging from 2 to 5;
- and X is an exchangeable anion;
- (b) passing said biological composition through said column;
- and,
- (c) recovering the separated components.
A liquid chromatographic column comprising an elongated tube, a strong anion exchange composition packed into said tube, said strong anion exchange composition comprising an inert porous particle having a size of about 5 to 20 microns and having attached to the surface of said inert porous particle by covalent bonding a silyl alkyl ammonium compound having the general formula:
- ##STR5## wherein R is methyl or ethyl;
- n is an integer ranging from 2 to 5;
- and X is an exchangeable anion, said strong anion exchange composition being capable of simultaneous separation of mixtures of polyfunctional compounds and isomers thereof.
A method for performing liquid chromatography comprising placing a solution of a composition comprising a mixture of polyfunctional compounds and isomers thereof on the head of a chromatographic column, flowing a solvent system through said chromatographic column, wherein said chromatographic column is packed with a packing material comprising a strong anion exchange composition, said strong anion exchange composition comprising an inert porous particle having a size from about 3 to 20 microns which has covalently attached to its surface a silyl alkyl ammonium compound having the general formula:
- ##STR6## wherein R is methyl or ethyl;
- n is an integer ranging from 2 to 5;
- and X is an exchangeable anion;
- wherein said strong anion exchange composition is capable of simultaneous separation of mixtures of polyfunctional compounds and isomers thereof.
A method for the separation of nucleotide isomers on a chromatographic column, the steps of the method comprising:
- (a) packing a column with a strong anion exchange composition comprising a microparticulate silica having a particle size of about 3 to about 20 microns which has covalently attached to the silica surface a silyl alkyl ammonium compound having the general formula:
- ##STR7## wherein R is methyl or ethyl;
- n is an integer ranging from 2 to 5;
- and X is an exchangeable anion;
- (b) passing said nucleotide isomer composition through said chromatographic column;
- and
- (c) recovering the separated components.
Dependencies are inferred from the claim text.