High speed preparative countercurrent chromatography with a multiple layer coiled column
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorYoichiro Ito
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Abstract
A horizontal flow-through apparatus for two-phase countercurrent chromatography consisting of a horizontal multi-layer helically coiled column carried on a flanged reel which is rotated about its own axis and simultaneously revolved around a stationary central parallel horizontal pipe at the same angular velocity and in the same direction to prevent twisting of its inlet and outlet flow tubes, which extend through the stationary central pipe. The multi-layer array can retain a large volume of the stationary phase against a high flow rate of the mobile phase, enabling the separation of the sample components to be completed in a relatively short period of time.
Claims13
An apparatus for continuous countercurrent chromatography comprising a support, helical separation column means including means to retain a large volume of stationary phase comprising a multi-layer helically coiled column rotatably mounted on the support on a first axis, inlet and outlet flow tubes connected to the terminal ends of said multi-layer coiled column, and means to simultaneously rotate said multi-layer coiled column around said first axis and revolve it around a second axis spaced from and being parallel to said first axis, whereby to simultaneously develop both force reversals and centrifugal forces in said multi-layer coiled column.
Dependencies are inferred from the claim text.