Immunoassay of thymosin .alpha..sub.1
AssigneeHoffmann-La Roche Inc.
InventorsEdward Newman, A. Hirotoshi Nishikawa, Herbert E. Spiegel, Julia Symington
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Abstract
An improved immunoassay for the polypeptide hormone thymosin .alpha..sub.1 is described. The assay employs an improved antibody which is elicited by an immunogen which has been prepared by coupling [Tyr.sup.1 ]-thymosin .alpha..sub.1 to an immunogenic carrier protein using a bifunctional diazonium coupling agent.
Claims16
In a method for the assay of thymosin .alpha..sub.1 in a sample, which method comprises mixing said sample with a known amount of labelled thymosin .alpha..sub.1 and an antibody which will selectively complex with said thymosin .alpha..sub.1, separating the resulting antibody-antigen complex from uncomplexed labelled thymosin .alpha..sub.1, measuring the degree of binding of the said labelled thymosin .alpha..sub.1 in said complex and determining the amount of thymosin .alpha..sub.1 present in said sample by comparing said degree of binding to a standard curve, the improvement which comprises employing as the antibody therein an antibody which has been elicited in a mammal in response to an immunogen comprising [Tyr.sup.1 ]-thymosin .alpha..sub.1 coupled to an immunogenic carrier protein.
An immunogen for eliciting antibody production which comprises [Tyr.sup.1 ]-thymosin .alpha..sub.1 coupled to an immunogenic carrier protein.
An antibody to thymosin .alpha..sub.1 which is elicited in a mammal in response to an immunogen comprising [Tyr.sup.1 ]-thymosin .alpha..sub.1 coupled to an immunogenic carrier protein.
Dependencies are inferred from the claim text.