Process for separation of carbohydrates
AssigneeSeikagaku Kogyo Kabushiki Kaisha
InventorsKinzo Nagasawa, Akira Ogamo
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Abstract
There is provided a process for the separation of carbohydrates by use of hydrophobic interaction chromatography. The process according to the present invention can accomplish effective separation of carbohydrates, particularly mucopolysaccharides including heparin, heparan sulfate, chondroitin sulfates, dermatan sulfate, hyaluronic acid, keratan sulfate and chondroitin-polysulfates. As the hydrophobic ligand in the chromatographic support to be used in the present invention, there may particularly be mentioned and alkyl group; an alkyl group substituted with a hydroxyl group, a carboxyl group and/or an amino group; an aryl group; or an aralkyl group. The chromatographic support is selected from cross-linked and non-cross-linked agaroses and polyvinyl alcohols.
Claims12
A process for separation of mucopolysaccharides which comprises separating a mucopolysaccharide into its constituent components comprising
- contacting said mucopolysaccharide with a chromatographic column comprising a support selected from the group consisting of crosslinked agarose, non-cross-linked agarose, and a polyvinyl alcohol, said support containing hydrophobic ligands selected from the group consisting of alkyl groups;
- alkyl groups substituted with at least one group selected from the group consisting of a hydroxyl group, a carboxyl group and an amino group;
- alkylene groups substituted with a hydroxyl group;
- aryl groups;
- and alalkyl groups, and thereby separating said mucopolysaccharide into its constituent components.
Dependencies are inferred from the claim text.