Fluorescent polarization immunoassay utilizing substituted triazinylaminofluoresceins
AssigneeAbbott Laboratories
InventorsChao-Huei J. Wang, Stephen D. Stroupe, Michael E. Jolley
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Abstract
An immunochemical method and reagent compounds for determining ligands in a sample. The reagent compounds are derivatives of a triazinylaminofluorescein and are represented by the structural formula ##STR1## wherein Y is halo or lower alkyl; and R is a ligand-analog wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody. The reagent compound and an antibody specific to the ligand are added to the sample in a fluorescence polarization immunoassay.
Claims34
A method for determining ligands in a sample comprising intermixing with said sample a biologically acceptable salt of a tracer of the formula:
- ##STR37## wherein Y is halo or lower alkyl;
- and
- R is a ligand-analog wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody;
- and an antibody capable of specifically recognizing said ligand and said tracer;
- and then determining the amount of tracer bound to antibody by fluorescence polarization techniques as a measure of the amount of ligand in the sample.
Dependencies are inferred from the claim text.