Silver stain procedure and kit for same
AssigneeThe Upjohn Company
InventorsLonnie D. Adams, David W. Sammons
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Abstract
A silver stain procedure wherein a substance capable of binding silver is treated with a glutaraldehyde solution, an aqueous silver salt solution, a reducing solution and an aqueous carbonate or sulfate solution and kit useful in practicing same.
Claims13
A method for staining a substance which is capable of binding silver which is supported in a matrix which comprises the steps of treating said substance with a 2.5-25% glutaraldehyde solution, washing said substance with water, equilibrating said substance in an aqueous silver salt solution, subjecting said substance to a reducing solution, and immersing said substance in multiple and sequential aqueous carbonate or sulfate salt solutions.
A method for staining a protein, polypeptide or protein containing substance which is supported in a matrix which comprises the steps of treating said substance with a 2.5-25% glutaraldehyde solution for one to 6 hours, washing said substance one to 6 hours with distilled water, equilibrating said substance for about two hours in an aqueous silver nitrate solution containing from about 0.5 to 6 grams of silver salts per liter of water, then subjecting said substance for about 30 seconds to 5 minutes to a reducing solution comprising about 0.5 to 2 ml of 37% formalin per about 150 ml of 0.5 to 1.0 N aqueous sodium hydroxide followed by immersing said substance in 3 sequential solutions of 0.075 to 1.5% aqueous sodium carbonate for about one to 5 minutes each.
A mercantile kit comprising multiple containers separately holding a concentrated solution of glutaraldehyde, a silver salt, a base solution, an aldehyde solution and a carbonate or sulfate salt.
Dependencies are inferred from the claim text.