Electrophoretic separation of isoenzymes utilizing a stable polyacrylamide system
AssigneeTechAmerica Group, Inc.
InventorsAurora F. de Castro, Surendra K. Gupta
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Abstract
A method for separating isoenzymes is disclosed. The method involves polyacrylamide gel electrophoresis in a buffer solution of a salt of 2-amino-2-methyl-1,3-propanediol at a pH of about 6.4 to 7.3 and an electrolyte buffer of 2-amino-2-methyl-1,3-propanediol taurine at a pH of about 8.0 to 10.0. After separation of the isoenzymes, the amount of individual isoenzymes present is determined.
Claims7
A method for separating isoenzymes which comprises the steps of applying an isoenzyme-containing sample to a support medium of polyacryl-amide gel containing acrylamide monomers in a buffer solution of a salt of 2-amino-2-methyl-1,3-propanediol at a pH of about 6.4 to 7.3 and an electrolyte buffer of 2-amino-2-methyl-1,3-propanediol taurine at a pH of about 8.0 to 10.0, subjecting the mixture to a differential electrical potential to produce migration of the isoenzymes and buffer ions and determining the presence of separated isoenzymes.
Dependencies are inferred from the claim text.